On the Ca2 + binding and conformational change in EF-hand domains: Experimental evidence of Ca2 +-saturated intermediates of N-domain of calmodulin

Article


Ababou, A., Zaleska, Mariola and Pfuhl, Mark 2017. On the Ca2 + binding and conformational change in EF-hand domains: Experimental evidence of Ca2 +-saturated intermediates of N-domain of calmodulin. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1865 (6), pp. 640-651. https://doi.org/10.1016/j.bbapap.2017.03.003
AuthorsAbabou, A., Zaleska, Mariola and Pfuhl, Mark
Abstract

Double mutation of Q41L and K75I in the N-domain of calmodulin (N-Cam) stabilizes the closed form of N-Cam such that binding of Ca2 + in solution no longer triggers a conformational change to the open form, and its Ca2 + binding affinity decreases dramatically. To further investigate the solvation effects on the structure, Ca2 + binding affinity and conformational dynamics of this N-Cam double mutant in the Ca2 + saturated state, we solved its X-ray structure. Surprisingly, the structure revealed an open conformation of the domain which contradicts its closed conformation in solution. Here we provide evidence that crystallization conditions were responsible for this Ca2 +-saturated domain open conformation in the crystal. Importantly, we demonstrate that the presence of the crystallization co-precipitant and alcohols were able to induce a progressive opening of the closed form of this domain, in Ca2 + saturated state, in solution. However, in the Ca2 + depleted state, addition of alcohols was unable to induce any opening of this domain in solution. In addition, in the Ca2 + saturated state, the molecular dynamics simulations show that while N-Cam can populate the open and closed conformation, the N-Cam double mutant exclusively populates the closed conformation. Our results provide experimental evidence of intermediate conformations of Ca2 +-saturated N-Cam in solution. We propose that conformational change of Ca2 + sensor EF-hand domains depends on solvation energetics, Ca2 + binding to promote the full open form, Ca2 + depleted state conformational dynamics, and the chemical properties of the molecules nearby key residues such as those at positions 41 and 75 in N-Cam.

KeywordsEF-hand domain; Ca2 + binding; conformational change; solvation enegetics; X-ray crystallography; MD simulation
JournalBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics
Journal citation1865 (6), pp. 640-651
ISSN1570-9639
Year2017
PublisherElsevier
Accepted author manuscript
License
Digital Object Identifier (DOI)https://doi.org/10.1016/j.bbapap.2017.03.003
Publication dates
Print10 Mar 2017
Publication process dates
Deposited13 Mar 2017
Accepted09 Mar 2017
External resourceCrystal structure of double mutant of N-domain of human calmodulin
Copyright information© 2017 Elsevier
Permalink -

https://repository.uel.ac.uk/item/84ww0

Download files


Accepted author manuscript
On the Ca2 binding and.pdf
License: CC BY-NC-ND 3.0

  • 176
    total views
  • 451
    total downloads
  • 1
    views this month
  • 1
    downloads this month

Export as

Related outputs

New insights into the structural and functional involvement of the gate loop in AcrB export activity
Ababou, A. 2017. New insights into the structural and functional involvement of the gate loop in AcrB export activity. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1866 (2), pp. 242-253. https://doi.org/10.1016/j.bbapap.2017.11.003