The relationship between probiotics, prebiotics and bile acids and the impact on gut health
PhD Thesis
Adebola, Oluwakemi 2009. The relationship between probiotics, prebiotics and bile acids and the impact on gut health. PhD Thesis University of East London School of Health, Sport and Bioscience
Authors | Adebola, Oluwakemi |
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Type | PhD Thesis |
Abstract | Epidemiological studies show a diet rich in fats and processed meats is often associated with higher levels of secondary bile acids and carcinogens in the gut and increases the risk of colorectal diseases. In recent years the consumption of probiotics and prebiotics has been Lactulose was the prebiotic of choice for both L. acidophilus NCFM LYO 10 and L. reuteri with growth comparable to growth in glucose. Small increases in growth were observed for both cultures with lactobionic acid as substrate whereas other cultures could not effectively L acidophilus NCTC 1723 deconjugated taurocholic, glycocholic, taurochenodeoxycholic and taurodeoxycholic acid, no deconjugation was observed for other cultures. The presence of prebiotics impacted on BSH activity i.e. whilst 2.83xlO" 2 nmol/min (p<0.01) cholic acid was released with 2% inulin reduced BSH activity was observed in 2% lactulose with 0.66x10" 2 nmol/min cholic acid released. No growth was observed in 2% lactobionic acid. Comparism of L. acidophilus NCFM LYO 10 cytosolic proteome revealed 16 spots whose expressions were either up or down regulated by the presence of cholic or glycocholic acid. 9 Deoxycholic, glycolithocholic, lithocholic acid (0.5mM) and faecal water 50% (v/v) reduced the viability of HT29 cells by 75, 60, 74 and 50% respectively. Addition of prebiotics inulin and lactulose (0.5-2% w/v) resulted in a concentration dependent increase in the viability of cells exposed to deoxycholic acid by 110% and lithocholic acid by 20%. Similarly, cytotoxicity induced by faecal water was significantly (p<0.05) reduced by inulin (2.5%) and In conclusion, these findings suggest the protective benefits attributed to synbiotic applications in bile acid/salt stress are specific to the nature and type of bile acids with a rather complex physiological response being induced. Prebiotics exerts a concentration |
Keywords | Colorectal diseases; Gut health; Bile acids |
Year | 2009 |
Publication dates | |
Oct 2009 | |
Publication process dates | |
Deposited | 30 Oct 2013 |
Additional information | This thesis supplied via ROAR to UEL-registered users is protected by copyright and other intellectual property rights, and duplication of any part of the material is not permitted, except for your personal use for the purposes of non-commercial research and private study in electronic or print form. You must obtain permission from the copyright-holder for any other use. Electronic or print copies may not be offered, for sale or otherwise, to anyone. No quotation from the thesis may be published without proper acknowledgement. |
Publisher's version | File Access Level Registered users only |
https://repository.uel.ac.uk/item/86355
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