Karyotypic and 2C nuclear DNA in vitro induced off-types of East African highland banana (Musa AAA East Africa)
Msogoya, Theodosy, Grout, Brian and Roberts, Andy 2008. Karyotypic and 2C nuclear DNA in vitro induced off-types of East African highland banana (Musa AAA East Africa). Biotechnology. 7 (3), pp. 578-581.
|Authors||Msogoya, Theodosy, Grout, Brian and Roberts, Andy|
This study was conducted to determine chromosome number and 2C nuclear DNA content in tissue culture induced off-type banana (Musa AAA East Africa) landrace Uganda with tolerance black sigatoka disease, susceptibility to water stress, sparsely black-blotched pseudostems, taller pseudostems, late fruit maturation, altered inflorescence and higher fruit dry matter content. The off-type banana appeared to have higher (p<0.05) frequency of 31 and 32 chromosomes at 15.1 and 13.6%, respectively. Conversely, the frequency of 31 and 32 chromosomes was 12.0 and 9.6% for the micropropagation (MP) derived plants with no tissue culture history. Moreover, the off-type banana had lower (p<0.05) leaf 2C nuclear DNA amount of 1.72pg, whilst the MP and CP derived plants had 1.81 pg, respectively.
|Keywords||Choromosome number; 2C nuclear DNA amount; somaclonal variation; East African highland banana; Uganda; aneuploidy; flow cytometry; biotechnology|
|Journal citation||7 (3), pp. 578-581|
|Web address (URL)||http://hdl.handle.net/10552/192|
|Publication process dates|
|Deposited||12 Jun 2009|
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