Karyotypic and 2C nuclear DNA in vitro induced off-types of East African highland banana (Musa AAA East Africa)

Article


Msogoya, Theodosy, Grout, Brian and Roberts, Andy 2008. Karyotypic and 2C nuclear DNA in vitro induced off-types of East African highland banana (Musa AAA East Africa). Biotechnology. 7 (3), pp. 578-581.
AuthorsMsogoya, Theodosy, Grout, Brian and Roberts, Andy
Abstract

This study was conducted to determine chromosome number and 2C nuclear DNA content in tissue culture induced off-type banana (Musa AAA East Africa) landrace Uganda with tolerance black sigatoka disease, susceptibility to water stress, sparsely black-blotched pseudostems, taller pseudostems, late fruit maturation, altered inflorescence and higher fruit dry matter content. The off-type banana appeared to have higher (p<0.05) frequency of 31 and 32 chromosomes at 15.1 and 13.6%, respectively. Conversely, the frequency of 31 and 32 chromosomes was 12.0 and 9.6% for the micropropagation (MP) derived plants with no tissue culture history. Moreover, the off-type banana had lower (p<0.05) leaf 2C nuclear DNA amount of 1.72pg, whilst the MP and CP derived plants had 1.81 pg, respectively.

KeywordsChoromosome number; 2C nuclear DNA amount; somaclonal variation; East African highland banana; Uganda; aneuploidy; flow cytometry; biotechnology
JournalBiotechnology
Journal citation7 (3), pp. 578-581
ISSN1682-296X
Year2008
Publisher's version
License
CC BY-ND
Web address (URL)http://hdl.handle.net/10552/192
Publication dates
Print2008
Publication process dates
Deposited12 Jun 2009
Additional information

Citation:
Msogya T, Grout B, Roberts A, (2008) 'Karyotypic and 2C nuclear DNA in vitro induced off-types of East African highland banana (Musa AAA East Africa)' Biotechnology 7 (3) 578-581.

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