A Coprological Study of Endoparasites in the Icelandic Arctic Fox using Traditional and Molecular Methods

Masters Thesis


Evans, C. 2018. A Coprological Study of Endoparasites in the Icelandic Arctic Fox using Traditional and Molecular Methods. Masters Thesis University of East London School of Health, Sport and Bioscience
AuthorsEvans, C.
TypeMasters Thesis
Abstract

2019 dissertation for MRes. The Arctic fox (Vulpes lagopus) is the only land mammal native to the remote island of Iceland, which has
a history of contending with endemic parasites in canines. This dissertation utilises fox carcasses from
storage at the Icelandic Institute of Natural History to survey endoparasitic fauna, and comments on the
suitability of traditional and molecular methods for the identification of endoparasite species and
estimation of egg burden.
A total of 129 Arctic fox carcasses were analysed using traditional microscopy (Apacor Mini ParasepĀ®)
and molecular techniques (real-time PCR). Examination revealed 66.9% (n=77) of foxes had an
endoparasitic infection; Capillaria aerophila 6.9% (n=8), Diphyllobothrium latum 6.9% (n=8),
Toxascaris leonina 36.4% (n=41), Toxocara canis 9.3% (n=12), Toxoplasma gondii 4.7% (n=6), Eimeria or
Isospora 1.6% (n=2), and Cryptocotyle lingua 0.8% (n=1). All samples positive by PCR were detected by
microscopy.
The dissertation suggests that coprological analysis of frozen faeces using bright-field microscopy and
real-time PCR for the detection of parasites in wild Arctic fox populations in Iceland can be achieved
despite DNA degradation, interference with PCR inhibitors, and poor sample quality.

Year2018
PublisherUniversity of East London
Digital Object Identifier (DOI)doi:10.15123/uel.86zy5
File
License
File Access Level
Anyone
Publication dates
PrintDec 2018
Publication process dates
Deposited09 Sep 2019
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