Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh
Article
Solanki, Anisha, Yanez, Diana C., Ross, Susan, Lau, Ching-In, Papaioannou, Eleftheria, Li, Jiawei, Saldaña, J. and Crompton, Tessa 2018. Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh. Development. 145 (3), p. dev146910. https://doi.org/10.1242/dev.146910
Authors | Solanki, Anisha, Yanez, Diana C., Ross, Susan, Lau, Ching-In, Papaioannou, Eleftheria, Li, Jiawei, Saldaña, J. and Crompton, Tessa |
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Abstract | Gli3 is a Hedgehog (Hh)-responsive transcription factor that can function as a transcriptional repressor or activator. We show that Gli3 activity in mouse thymic epithelial cells (TECs) promotes positive selection and differentiation from CD4+ CD8+ to CD4+ CD8− single positive (SP4) cells in the fetal thymus and that Gli3 represses Shh. Constitutive deletion of Gli3, and conditional deletion of Gli3 from TECs, reduced differentiation to SP4, whereas conditional deletion of Gli3 from thymocytes did not. Conditional deletion of Shh from TECs increased differentiation to SP4, and expression of Shh was upregulated in the Gli3-deficient thymus. Use of a transgenic Hh reporter showed that the |
Journal | Development |
Journal citation | 145 (3), p. dev146910 |
ISSN | 0950-1991 |
Year | 2018 |
Publisher | The Company of Biologists |
Publisher's version | License CC BY |
Digital Object Identifier (DOI) | https://doi.org/10.1242/dev.146910 |
Web address (URL) | https://doi.org/10.1242/dev.146910 |
Publication dates | |
31 Jan 2018 | |
Publication process dates | |
Deposited | 11 Apr 2018 |
Accepted | 03 Jan 2018 |
Accepted | 03 Jan 2018 |
Funder | Medical Research Council |
Biotechnology and Biological Sciences Research Council | |
Wellcome Trust | |
Great Ormond Street Hospital | |
National Secretariat for Higher Education, Science, Technology and Innovation of Ecuador |
https://repository.uel.ac.uk/item/84908
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