Kisspeptin Prevention of Amyloid-β Peptide Neurotoxicityin Vitro

Article


Milton, Nathaniel G. N., Chilumuri, A., Rocha-Ferreira, Eridan, Nercessian, Amanda N. and Ashioti, Maria 2012. Kisspeptin Prevention of Amyloid-β Peptide Neurotoxicityin Vitro. ACS Chemical Neuroscience. 3 (9), pp. 706-719.
AuthorsMilton, Nathaniel G. N., Chilumuri, A., Rocha-Ferreira, Eridan, Nercessian, Amanda N. and Ashioti, Maria
Abstract

Alzheimer’s disease (AD) onset is associated with changes in hypothalamic-pituitary–gonadal (HPG) function. The 54 amino acid kisspeptin (KP) peptide regulates the HPG axis and alters antioxidant enzyme expression. The Alzheimer’s amyloid-β (Aβ) is neurotoxic, and this action can be prevented by the antioxidant enzyme catalase. Here, we examined the effects of KP peptides on the neurotoxicity of Aβ, prion protein (PrP), and amylin (IAPP) peptides. The Aβ, PrP, and IAPP peptides stimulated the release of KP and KP 45–54. The KP peptides inhibited the neurotoxicity of Aβ, PrP, and IAPP peptides, via an action that could not be blocked by kisspeptin-receptor (GPR-54) or neuropeptide FF (NPFF) receptor antagonists. Knockdown of KiSS-1 gene, which encodes the KP peptides, in human neuronal SH-SY5Y cells with siRNA enhanced the toxicity of amyloid peptides, while KiSS-1 overexpression was neuroprotective. A comparison of the catalase and KP sequences identified a similarity between KP residues 42–51 and the region of catalase that binds Aβ. The KP peptides containing residues 45–50 bound Aβ, PrP, and IAPP, inhibited Congo red binding, and were neuroprotective. These results suggest that KP peptides are neuroprotective against Aβ, IAPP, and PrP peptides via a receptor independent action involving direct binding to the amyloid peptides.

JournalACS Chemical Neuroscience
Journal citation3 (9), pp. 706-719
ISSN1948-7193
Year2012
PublisherElsevier
Digital Object Identifier (DOI)doi:10.1021/cn300045d
Web address (URL)https://doi.org/10.1021/cn300045d
Publication dates
Print30 May 2012
Publication process dates
Deposited09 Aug 2017
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