Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection

Article


Srey, M. T., Taccogna, A., Oksov, Y., Lustigman, S., Tai, P., Acord, J., Selkirk, M. E., Lamb, T. J. and Guiliano, D. 2020. Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. PLoS Neglected Tropical Diseases. 14 (Art. e0008842). https://doi.org/10.1371/journal.pntd.0008842
AuthorsSrey, M. T., Taccogna, A., Oksov, Y., Lustigman, S., Tai, P., Acord, J., Selkirk, M. E., Lamb, T. J. and Guiliano, D.
Abstract

Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned proteins have shown that some ESPs are potent immunogens capable of eliciting protective immune responses. Here we describe two novel proteins, Secreted from Muscle stage Larvae SML-4 and SML-5 which are 15 kDa and 12 kDa respectively. The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. While SML-5 is only expressed in mL1 and early stages of adult nematode development, SML-4 is a tyvosylated glycoprotein also produced by adult nematodes, indicating it may have a function in the enteral phase of the infection. Vaccination with these proteins resulted in an impaired establishment of adult stages and consequently a reduction in the burden of mL1 in BALB/c mice. This suggests that both proteins may be important for establishment of parasite infection of the intestine and are prophylactic vaccine candidates.

JournalPLoS Neglected Tropical Diseases
Journal citation14 (Art. e0008842)
ISSN1935-2735
Year2020
PublisherPublic Library of Science
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Anyone
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Digital Object Identifier (DOI)https://doi.org/10.1371/journal.pntd.0008842
Publication dates
Online18 Nov 2020
Publication process dates
Accepted14 Sep 2020
Deposited24 Nov 2020
FunderNational Institutes of Health
University of East London
Wellcome Trust
Copyright holder© 2020 The Authors
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