Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59

Article


Xu, Zhen, Misra, Raju, Jamrozy, Dorota, Paterson, Gavin K., Cutler, Ronald R., Holmes, Mark A., Gharbia, Saheer and Mkrtchyan, H. 2018. Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59. G3: Genes|Genomes|Genetics. 8 (7), pp. 2225-2230.
AuthorsXu, Zhen, Misra, Raju, Jamrozy, Dorota, Paterson, Gavin K., Cutler, Ronald R., Holmes, Mark A., Gharbia, Saheer and Mkrtchyan, H.
Abstract

Staphylococcus epidermidis is a major opportunistic pathogen primarily recovered from device-associated healthcare associated infections (DA-HAIs). Although S. epidermidis and other coagulase-negative staphylococci (CoNS) are less virulent than Staphylococcus aureus, these bacteria are an important reservoir of antimicrobial resistance genes and resistance-associated mobile genetic elements that can be transferred between staphylococcal species. We report a whole genome sequence of a multidrug resistant S. epidermidis (strain G6_2) representing multilocus sequence type (ST) 59 and isolated from an environmental sampling of a hotel room in London, UK. The genome of S. epidermidis G6_2 comprises of a 2408357 bp chromosome and six plasmids, with an average G+C content of 32%. The strain displayed a multi-drug resistance phenotype which was associated with carriage of 7 antibiotic resistance genes (blaZ, mecA, msrA, mphC, fosB, aacA-aphD, tetK) as well as resistance-conferring mutations in fusA and ileS. Antibiotic resistance genes were located on plasmids and chromosome. Comparative genomic analysis revealed that antibiotic resistance gene composition found in G6_2 was partly preserved across the ST59 lineage.

JournalG3: Genes|Genomes|Genetics
Journal citation8 (7), pp. 2225-2230
ISSN2160-1836
Year2018
PublisherGenetics Society of America: G3
Accepted author manuscript
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Digital Object Identifier (DOI)doi:10.1534/g3.118.200314
Web address (URL)https://doi.org/10.1534/g3.118.200314
Publication dates
Online30 Apr 2018
Print01 Jul 2018
Publication process dates
Deposited21 Jun 2018
Accepted23 Apr 2018
Accepted23 Apr 2018
FunderMedical Research Council
External resourceSupplemental Material for Xu et al., 2018
Copyright information© 2018 The authors
LicenseCC BY 4.0
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