Novel Methicillin-Resistant Staphylococcus aureus CC8 Clone Identified in a Hospital Setting in Armenia

Article


Jamrozy, D., Misra, R., Xu, Z., Ter-Stepanyan, M. M., Kocharyan, K. S., Cave, R., Dz Hambardzumyan, A. and Mkrtchyan, H. 2019. Novel Methicillin-Resistant Staphylococcus aureus CC8 Clone Identified in a Hospital Setting in Armenia. Frontiers in Microbiology. 10 (Art. 1592).
AuthorsJamrozy, D., Misra, R., Xu, Z., Ter-Stepanyan, M. M., Kocharyan, K. S., Cave, R., Dz Hambardzumyan, A. and Mkrtchyan, H.
Abstract

Whole-genome sequencing (WGS) of methicillin-resistant Staphylococcus aureus (MRSA) has been sparse in low- and middle-income countries, therefore, its population structure is unknown for many regions. We conducted a pilot surveillance of MRSA in the maternity ward of a teaching hospital in Armenia, to characterize the genotypes of circulating MRSA clones. In total, 10 MRSA isolates from a hospital environment (n = 4) and patients (n = 6) were recovered between March and May 2015 and April and May 2016, respectively. WGS analysis showed that the isolates belonged to two clonal complexes (CCs): CC8 (n = 8) and CC30 (n = 2). MRSA CC30 isolates carried staphylococcal cassette chromosome mec (SCCmec) type IVa, whereas MRSA CC8 revealed a type-Vᴛ-related SCCmec, which contained a CRISPR/Cas array and showed a high similarity to SCCmec found in coagulase-negative staphylococci. All but one MRSA CC8 isolates carried a plasmid identical to the pSK67 and four also carried a pathogenicity island similar to SaPI5. Phylogenetic analysis showed that the MRSA CC8 isolates formed a monophyletic cluster, which emerged around 1995 and was distinct from representatives of globally-distributed MRSA CC8 lineages. WGS characterization of MRSA in countries with no previous S. aureus genomic surveillance can therefore reveal an unrecognized diversity of MRSA lineages.

JournalFrontiers in Microbiology
Journal citation10 (Art. 1592)
ISSN1664-302X
Year2019
PublisherFrontiers Media
Publisher's version
License
File Access Level
Anyone
Supplemental file
File Access Level
Anyone
Digital Object Identifier (DOI)doi:10.3389/fmicb.2019.01592
Web address (URL)https://doi.org/10.3389/fmicb.2019.01592
Publication dates
Online10 Jul 2019
Publication process dates
Accepted26 Jun 2019
Deposited10 Jul 2019
FunderUniversity of East London
Wellcome Trust
Copyright holder© 2019 The Authors.
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