Detection of diverse genotypes of Methicillin-resistant Staphylococcus aureus from hospital personnel and the environment in Armenia

Article


Mkrtchyan, H., Xu, Zhen, Yacoub, Maria, Ter-Stepanyan, Mary M., Karapetyan, Hayk D., Kearns, Angela M., Cutler, Ronald R., Pichon, Bruno and Hambardzumyan, Armen Dz 2017. Detection of diverse genotypes of Methicillin-resistant Staphylococcus aureus from hospital personnel and the environment in Armenia. Antimicrobial Resistance & Infection Control. 6, p. Art. 19.
AuthorsMkrtchyan, H., Xu, Zhen, Yacoub, Maria, Ter-Stepanyan, Mary M., Karapetyan, Hayk D., Kearns, Angela M., Cutler, Ronald R., Pichon, Bruno and Hambardzumyan, Armen Dz
Abstract

Background

Methicillin-resistant Staphylococcus aureus (MRSA) is a public health concern internationally. Studies examining a range of cohorts have been reported from various regions of the world, but little is known about the molecular epidemiology of MRSA in Armenia.
Methods

Between May and September 2013, twenty isolates of methicillin-resistant Staphylococcus aureus (MRSA; mecA positive) were recovered from hospital personnel (n = 10; 9 females, 1 male) and environmental sites (n = 10) in the maternity ward of one of the teaching hospitals in Armenia.
Results

Multi-locus sequence type clonal complex (MLST-CC) assignments inferred from spa typing data revealed the majority belonged to 3 pandemic lineages of MRSA including: t008-CC8-SCCmecV (n = 10; 7 from personnel); t021-CC30-SCCmecIV (n = 5; all environmental); and t1523-CC45 (n = 2; 1 from personnel), one harboured SCCmecV the other was SCCmec non-typable. The remainder identified as belonging to genotype t364-CC182, both of which harboured a novel SCCmec cassette with kdp, rif5, ccrB2 and ccrC detected by PCR (both from personnel); and t325-CC88-SCCmecIV (n = 1; environmental). All MRSA were negative for the Panton-Valentine Leukocidin (PVL) locus and three CC8 strains were positive for the arginine catabolic element (ACME).
Conclusions

In this small study, we report for the first time of the occurrence of diverse MRSA genotypes belonging to both pandemic and more sporadic international clones in Armenia harbouring the smaller SCCmec types and/or ACME, both of which have been associated with strain fitness. Further surveillance is warranted to better understand the prevalence, clinical and molecular epidemiology of MRSA throughout Armenia.

JournalAntimicrobial Resistance & Infection Control
Journal citation6, p. Art. 19
ISSN2047-2994
Year2017
PublisherBMC
Publisher's version
License
Digital Object Identifier (DOI)doi:10.1186/s13756-017-0169-0
Web address (URL)https://doi.org/10.1186/s13756-017-0169-0
Publication dates
Print07 Feb 2017
Publication process dates
Deposited11 Jan 2019
Accepted03 Jan 2017
Accepted03 Jan 2017
FunderSociety of Applied Microbiology
Society of Applied Microbiology
Copyright information© 2017 The authors
Permalink -

https://repository.uel.ac.uk/item/84x2x

  • 7
    total views
  • 27
    total downloads
  • 2
    views this month
  • 8
    downloads this month

Related outputs

Whole genome sequencing revealed new molecular characteristics in multidrug resistant staphylococci recovered from high frequency touched surfaces in London
Cave, R., Misra, R., Chen, J., Wang, S. and Mkrtchyan, H. 2019. Whole genome sequencing revealed new molecular characteristics in multidrug resistant staphylococci recovered from high frequency touched surfaces in London. Scientific Reports. 9 (Art. 9637).
Novel Methicillin-Resistant Staphylococcus aureus CC8 Clone Identified in a Hospital Setting in Armenia
Jamrozy, D., Misra, R., Xu, Z., Ter-Stepanyan, M. M., Kocharyan, K. S., Cave, R., Dz Hambardzumyan, A. and Mkrtchyan, H. 2019. Novel Methicillin-Resistant Staphylococcus aureus CC8 Clone Identified in a Hospital Setting in Armenia. Frontiers in Microbiology. 10 (Art. 1592).
The prevalence, antibiotic resistance and mecA characterization of coagulase negative staphylococci recovered from non-healthcare settings in London, UK
Xu, Zhen, Shah, Haroun N., Misra, Raju, Chen, Jiazhen, Zhang, Wenhong, Liu, Yuting, Cutler, Ronald R. and Mkrtchyan, H. 2018. The prevalence, antibiotic resistance and mecA characterization of coagulase negative staphylococci recovered from non-healthcare settings in London, UK. Antimicrobial Resistance & Infection Control. 7 (73).
Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59
Xu, Zhen, Misra, Raju, Jamrozy, Dorota, Paterson, Gavin K., Cutler, Ronald R., Holmes, Mark A., Gharbia, Saheer and Mkrtchyan, H. 2018. Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59. G3: Genes|Genomes|Genetics. 8 (7), pp. 2225-2230.