Comparative Proteomic Profiling of Methicillin‐Susceptible and Resistant Staphylococcus aureus
Article
Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R. and Mkrtchyan, H. 2019. Comparative Proteomic Profiling of Methicillin‐Susceptible and Resistant Staphylococcus aureus. Proteomics. 20 (Art. 1900221). https://doi.org/10.1002/pmic.201900221
Authors | Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R. and Mkrtchyan, H. |
---|---|
Abstract | Purpose Staphylococcus aureus is a highly successful human pathogen responsible for wide range of infections. In this study, we provide insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin susceptible and methicillin resistant Staphylococcus aureus (MSSA; MRSA) recovered from non‐healthcare environments. Experiment design Three environmental MSSA and three environmental MRSA were selected for proteomic profiling using iTRAQ MS/MS. Gene Ontology (GO) Annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Annotation were applied to interpret the functions of the proteins detected. Results 792 proteins were identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA revealed that 8 of out 792 proteins were up‐regulated and 156 were down‐regulated. Proteins that had differences in abundance were predominantly involved in catalytic and binding activity. Among 164 differently abundant proteins, 29 were involved in pathogenesis, antimicrobial resistance, stress response, mismatch repair and cell wall synthesis. Twenty‐two proteins associated with pathogenicity, including spa, sbi, clfA and dlt were up‐regulated in MRSA. Moreover, the up‐regulated pathogenic protein entC2 in MSSA was determined to be a super antigen potentially capable of triggering toxic shock syndrome in the host. Conclusions Enhanced pathogenicity, antimicrobial resistance and stress response were observed in MRSA compared to MSSA. |
Journal | Proteomics |
Journal citation | 20 (Art. 1900221) |
ISSN | 1615-9853 |
Year | 2019 |
Publisher | Wiley |
Accepted author manuscript | License File Access Level Anyone |
Supplemental file | File Access Level Anyone |
Supplemental file | File Access Level Anyone |
Digital Object Identifier (DOI) | https://doi.org/10.1002/pmic.201900221 |
Web address (URL) | https://doi.org/10.1002/pmic.201900221 |
Publication dates | |
Online | 24 Dec 2019 |
16 Jan 2020 | |
Publication process dates | |
Accepted | 20 Dec 2019 |
Deposited | 06 Jan 2020 |
Copyright holder | © 2019 Wiley. |
Copyright information | This is the peer reviewed version of the following article: Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R., Mkrtchyan, H. V., Comparative Proteomic Profiling of Methicillin‐Susceptible and Resistant Staphylococcus aureus. Proteomics 2020, 20, 1900221. https://doi.org/10.1002/pmic.201900221. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
https://repository.uel.ac.uk/item/876xy
Download files
Accepted author manuscript
Xu_et_al-2019-PROTEOMICS.pdf | ||
License: Wiley Terms and Conditions for Use of Self-Archived Versions | ||
File access level: Anyone |
Supplemental file
pmic13243-sup-0001-suppmats1-1.docx | ||
File access level: Anyone |
pmic13243-sup-0002-suppmats2.xlsx | ||
File access level: Anyone |
134
total views138
total downloads0
views this month0
downloads this month