Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways

Article


Piovezan, Anna P., Batisti, Ana P., Benevides, Maria L.A.C.S., Turnes, Bruna L., Martins, Daniel F., Kanis, Luiz, Duarte, Elisa C.W., Cavalheiro, Alberto J., Bueno, Paula C.P., Seed, M., Norling, Lucy V., Cooper, Dianne, Headland, Sarah, Souza, Patrícia R.P.S. and Perretti, Mauro 2017. Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways. Journal of Ethnopharmacology. 204, pp. 179-188.
AuthorsPiovezan, Anna P., Batisti, Ana P., Benevides, Maria L.A.C.S., Turnes, Bruna L., Martins, Daniel F., Kanis, Luiz, Duarte, Elisa C.W., Cavalheiro, Alberto J., Bueno, Paula C.P., Seed, M., Norling, Lucy V., Cooper, Dianne, Headland, Sarah, Souza, Patrícia R.P.S. and Perretti, Mauro
Abstract

Ethnopharmacological relevance

Casearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain.

Aim of the study

The present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP).

Methods and results

Male Swiss mice were subjected to ischemia of the right hind paw (3 h), then reperfusion was allowed. At 10 min, 24 h or 48 h post-ischemia/reperfusion (I/R), different groups of animals were treated with HCE-CS (30 mg/Kg, orally [p.o]), selected agonists at the proresolving receptor ALX/FPR2 (natural molecules like resolvin D1 and lipoxin A4 or the synthetic compound BML-111; 0.1–1 µg/animal) or vehicle (saline, 10 mL/Kg, s.c.), in the absence or presence of the antagonist WRW4 (10 µg, s.c.). Mechanical hyperalgesia (paw withdrawal to von Frey filament) was asseseed together with histological and immunostainning analyses. In these settings, pro-resolving mediators reduced mechanical hyperalgesia and HCE-CS or BML-111 displayed anti-hyperalgesic effects which was markedly attenuated in animals treated with WRW4. ALX/FPR2 expression was raised in skeletal muscle or neutrophils after treatment with HCE-CS or BML-111.

Conclusion

These results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2.

KeywordsCasearia sylvestris; Salicaceae; Chronic post-ischemia pain; Inflammation; ALX/FPR2
JournalJournal of Ethnopharmacology
Journal citation204, pp. 179-188
ISSN0378-8741
1872-7573
Year2017
PublisherElsevier for International Society for Ethnopharmacology
Accepted author manuscript
License
CC BY-NC-ND
Digital Object Identifier (DOI)doi:10.1016/j.jep.2017.03.059
Publication dates
Print13 Apr 2017
Publication process dates
Deposited02 May 2017
Accepted31 Mar 2017
Accepted31 Mar 2017
FunderCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
FAPESC - Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina
UNISUL (Brazil)
European Union's Seventh Framework Programme
CoFund (Marie Curie Actions; WHRI Academy)
PIBIC-CNPq/UNISUL
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina
Universidade do Sul de Santa Catarina
Seventh Framework Programme
Research Executive Agency
PIBIC-CNPq/UNISUL
Copyright information© 2017 Elsevier
LicenseCC BY-NC-ND 3.0
Permalink -

https://repository.uel.ac.uk/item/84w22

  • 7
    total views
  • 35
    total downloads
  • 2
    views this month
  • 11
    downloads this month

Related outputs

High-intensity interval training and moderate-intensity continuous training in adults with Crohn’s disease: a pilot randomised controlled trial
Tew, Garry A., Leighton, Dean, Carpenter, R., Anderson, Simon, Langmead, Louise, Ramage, John, Faulkner, James, Coleman, Elizabeth, Fairhurst, Caroline, Seed, M. and Bottoms, Lindsay 2019. High-intensity interval training and moderate-intensity continuous training in adults with Crohn’s disease: a pilot randomised controlled trial. BMC Gastroenterology. 19, p. Art. 19.
Feasibility of high-intensity interval training and moderate-intensity continuous training in adults with inactive or mildly active Crohn’s disease: study protocol for a randomised controlled trial
Tew, Garry A., Carpenter, R., Seed, M., Anderson, Simon, Langmead, Louise, Fairhurst, Caroline and Bottoms, Lindsay 2017. Feasibility of high-intensity interval training and moderate-intensity continuous training in adults with inactive or mildly active Crohn’s disease: study protocol for a randomised controlled trial. Pilot and Feasibility Studies. 2017 (3:17).
Applying refinement to the use of mice and rats in rheumatoid arthritis research
Hawkins, Penny, Armstrong, Rachel, Boden, Tania, Garside, Paul, Knight, Katherine, Lilley, Elliot, Seed, M., Wilkinson, Michael and Williams, Richard O. 2015. Applying refinement to the use of mice and rats in rheumatoid arthritis research. Inflammopharmacology. 23 (4), pp. 131-150.
Does this case hold the answer to one of the worse types of pain in medicine--that of loin pain haematuria syndrome (LPHS)
Russell, Alan, Chatterjee, Suman and Seed, M. 2015. Does this case hold the answer to one of the worse types of pain in medicine--that of loin pain haematuria syndrome (LPHS). BMJ Case Reports. 2015 (apr26).
Animal models of rheumatoid arthritis: How informative are they?
McNamee, Kay, Williams, Richard and Seed, M. 2015. Animal models of rheumatoid arthritis: How informative are they? European Journal of Pharmacology. 759, pp. 278-286.
Targeting of viral interleukin-10 with an antibody fragment specific to damaged arthritic cartilage improves its therapeutic potency
Hughes, Chris, Sette, Angelica, Seed, M., D’Acquisto, Fulvio, Manzo, Antonio, Vincent, Tonia L, Lim, Ngee and Nissim, Ahuva 2014. Targeting of viral interleukin-10 with an antibody fragment specific to damaged arthritic cartilage improves its therapeutic potency. Arthritis Research & Therapy. 16 (4), p. R151.