Evaluating thermogravimetric analysis for the measurement of drug loading in mesoporous silica nanoparticles (MSNs)

Article


Almaghrabi, M., Alqurshi, A., Jadhav, S. A., Mazzacuva, F., Cilibrizzi, A., Raimi-Abraham, B. and Royall, P. G. 2023. Evaluating thermogravimetric analysis for the measurement of drug loading in mesoporous silica nanoparticles (MSNs). Thermochimica Acta. 730 (Art. 179616). https://doi.org/10.1016/j.tca.2023.179616
AuthorsAlmaghrabi, M., Alqurshi, A., Jadhav, S. A., Mazzacuva, F., Cilibrizzi, A., Raimi-Abraham, B. and Royall, P. G.
Abstract

In this study, a thermogravimetric analysis (TGA) method for measuring the drug loading in mesoporous silica nanoparticles (MSNs) has been developed and evaluated in comparison with the drug loading quantification by high-performance liquid chromatography (HPLC). Indapamide was loaded into two different types of MSNs, namely Mobile Crystalline Material (MCM-41, pore size = 1.2 nm) and Santa Barbara Amorphous (SBA-15, pore size = 4.1 nm). Physical mixtures of the drug and silica gave a linear correlation between the observed and expected drug content for both TGA and HPLC, which were used for calibration purposes. The limit of detection (LOD) for the TGA method obtained from the physical mixture calibration curve was 0.77 % (w/w) and the r² value was 0.9936, whereas the HPLC had a LOD of 0.06 % (w/w) and an r² value of 0.9933. The sensitivity of the TGA method was well established using the drug loading studies, as it can detect the low loading of MCM-41 at 2.2 ± 0.21 % (w/w), compared to 5.1 ± 0.12 % (w/w) with the SBA-15. In all samples applied, the multiple comparison analysis showed an insignificant difference between the two methods (p > 0.05). The TGA data presented good evidence for using this technique as a sensitive, cost-effective, and low-variable quantitative analysis in the drug loading determination of the MSNs. TGA is not a selective method of quantification, but optimising the method using the pure and blank samples of MSNs and drug can significantly improve the sensitivity. This work provides a unique approach to apply TGA as a selective and more favourable method to characterise MSNs to do early formulation developments.

JournalThermochimica Acta
Journal citation730 (Art. 179616)
ISSN0040-6031
Year2023
PublisherElsevier
Publisher's version
License
File Access Level
Anyone
Supplemental file
File Access Level
Anyone
Digital Object Identifier (DOI)https://doi.org/10.1016/j.tca.2023.179616
Publication dates
Online20 Oct 2023
PrintDec 2023
Publication process dates
Accepted07 Oct 2023
Deposited20 Nov 2023
FunderSaudi Arabian Cultural Bureau (SACB)
Copyright holder© 2023, The Authors
Permalink -

https://repository.uel.ac.uk/item/8wyv8

Download files


Publisher's version
1-s2.0-S0040603123001855-main.pdf
License: CC BY 4.0
File access level: Anyone


Supplemental file
1-s2.0-S0040603123001855-mmc1.pdf
File access level: Anyone

  • 27
    total views
  • 13
    total downloads
  • 9
    views this month
  • 4
    downloads this month

Export as

Related outputs

PDE4 Inhibitors: Profiling Hits through the Multitude of Structural Classes
Jian, J., Mazzacuva, F., Crocetti, L., Giovannoni, M. P. and Cilibrizzi, A. 2023. PDE4 Inhibitors: Profiling Hits through the Multitude of Structural Classes. International Journal of Molecular Sciences. 24 (14), p. 11518. https://doi.org/10.3390/ijms241411518
Optimization of 4-amino-pyridazin-3(2H)-one as a valid core scaffold for FABP4 inhibitors
Floresta, G., Crocetti, L., Rodrigues de Oliveria Silva, R., Patamia, V., Mazzacuva, F., Chen, Y. C. S., Vergelli, C. and Cilibrizzi, A. 2023. Optimization of 4-amino-pyridazin-3(2H)-one as a valid core scaffold for FABP4 inhibitors. Archiv der Pharmazie. In Press. https://doi.org/10.1002/ardp.202300314
OCT2013, an ischaemia-activated antiarrhythmic prodrug, devoid of the systemic side effects of lidocaine
Hesketh, L. M., Sikkel, M. B., Mahoney-Sanchez, L., Mazzacuva, F., Chowdury, R. A., Tzortzis, K. N., Firth, J., MacLeod, K. T., Ogrodzinski, S., Wilder, C. D. E., Pattersone, L. H., Peters, N. S. and Curtis, M. J. 2022. OCT2013, an ischaemia-activated antiarrhythmic prodrug, devoid of the systemic side effects of lidocaine. British Journal of Pharmacology. 179 (9), pp. 2037-2053. https://doi.org/10.1111/bph.15764
The surfactant co-formulant POEA in the glyphosate-based herbicide RangerPro but not glyphosate alone causes necrosis in Caco-2 and HepG2 human cell lines and ER stress in the ToxTracker assay
Mesnage, R., Gerguson, S., Brandsma, I., Moelijker, N., Zhang, G., Mazzacuva, F., Caldwell, A., Halket, J. and Antoniou, M. N. 2022. The surfactant co-formulant POEA in the glyphosate-based herbicide RangerPro but not glyphosate alone causes necrosis in Caco-2 and HepG2 human cell lines and ER stress in the ToxTracker assay. Food and Chemical Toxicology. 168 (Art. 113380). https://doi.org/10.1016/j.fct.2022.113380
Ligand Growing Experiments Suggested 4-amino and 4-ureido pyridazin-3(2H)-one as Novel Scaffold for FABP4 Inhibition
Crocetti, L., Floresta, G., Zagni, C., Merugu, D., Mazzacuva, F., Rodrigues de Oliveira Silva, R., Vergelli, C., Giovannoni, M. P. and Cilibrizzi, A. 2022. Ligand Growing Experiments Suggested 4-amino and 4-ureido pyridazin-3(2H)-one as Novel Scaffold for FABP4 Inhibition. Pharmaceuticals. 15 (11), p. 1335. https://doi.org/10.3390/ph15111335
Tissue Proteome of 2-Hydroxyacyl-CoA Lyase Deficient Mice Reveals Peroxisome Proliferation and Activation of ω-Oxidation
Khalil, Y., Carrino, S., Lin, F., Ferlin, A., Lad, H. V., Mazzacuva, F., Falcone, S., Rivers, N., Banks, G., Concas, D., Aguilar, C., Haynes, A. R., Blease, A., Nicol, T., Al-Shawi, R., Heywood, W., Potter, P., Mills, K., Gale, D. P. and Clayton, P. T. 2022. Tissue Proteome of 2-Hydroxyacyl-CoA Lyase Deficient Mice Reveals Peroxisome Proliferation and Activation of ω-Oxidation. International Journal of Molecular Sciences. 23 (Art. 987). https://doi.org/10.3390/ijms23020987
Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats
Mesnage, R., Teixeira, M., Mandrioli, D., Falcioni, L., Zwittink, D., Mazzacuva, F., Caldwell, A., Halket, J., Amiel, C., Panoff, J-M., Belpoggi, F. and Antoniou, M. N. 2021. Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. Environmental Health Perspectives. 129 (1). https://doi.org/10.1289/EHP6990
Urinary excretion of herbicide co-formulants after oral exposure to roundup MON 52276 in rats
Mesnage, R., Mazzacuva, F., Caldwell, A., Halket, J. and Antoniou, M. N. 2021. Urinary excretion of herbicide co-formulants after oral exposure to roundup MON 52276 in rats. International Journal of Environmental Research and Public Health. 197 (Art. 111103). https://doi.org/10.1016/j.envres.2021.111103