Galectin-1 is required for the regulatory function of B cells

Article


Alhabbab, R., Blair, P., Smyth, L., Ratnasothy, K., Peng, Q., Moreau, A., Lechler, R., Elgueta, R. and Lombardi, G. 2018. Galectin-1 is required for the regulatory function of B cells. Scientific Reports. 8 (2725).
AuthorsAlhabbab, R., Blair, P., Smyth, L., Ratnasothy, K., Peng, Q., Moreau, A., Lechler, R., Elgueta, R. and Lombardi, G.
Abstract

Galectin-1 (Gal-1) is required for the development of B cells in the bone marrow (BM), however very little is known about the contribution of Gal-1 to the development of B cell regulatory function. Here, we report an important role for Gal-1 in the induction of B cells regulatory function. Mice deficient of Gal-1 (Gal-1−/−) showed significant loss of Transitional-2 (T2) B cells, previously reported to include IL-10+ regulatory B cells. Gal-1−/− B cells stimulated in vitro via CD40 molecules have impaired IL-10 and Tim-1 expression, the latter reported to be required for IL-10 production in regulatory B cells, and increased TNF-α expression compared to wild type (WT) B cells. Unlike their WT counterparts, T2 and T1 Gal-1−/− B cells did not suppress TNF-α expression by CD4+ T cells activated in vitro with allogenic DCs (allo-DCs), nor were they suppressive in vivo, being unable to delay MHC-class I mismatched skin allograft rejection following adoptive transfer. Moreover, T cells stimulated with allo-DCs show an increase in their survival when co-cultured with Gal-1−/− T2 and MZ B cells compared to WT T2 and MZ B cells. Collectively, these data suggest that Gal-1 contributes to the induction of B cells regulatory function.

JournalScientific Reports
Journal citation8 (2725)
ISSN2045-2322
Year2018
PublisherNature Publishing Group
Publisher's version
License
Digital Object Identifier (DOI)doi:10.1038/s41598-018-19965-z
Web address (URL)https://doi.org/10.1038/s41598-018-19965-z
Publication dates
Online09 Feb 2018
Publication process dates
Deposited14 Feb 2018
Accepted04 Jan 2018
Accepted04 Jan 2018
FunderBritish Heart Foundation
Medical Research Council
National Institute for Health Research
Copyright information© 2018 The authors
LicenseCC BY 4.0
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https://repository.uel.ac.uk/item/848yz

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