Carbon nanotubes' surface chemistry determines their potency as vaccine nanocarriers in vitro and in vivo

Article

Hassan, Hatem A.F.M., Smyth, L., Rubio, Noelia, Ratnasothy, Kulachelvy, Wang, Julie T.-W., Bansal, Sukhvinder S., Summers, Huw D., Diebold, Sandra S., Lombardi, Giovanna and Al-Jamal, Khuloud T. 2016. Carbon nanotubes' surface chemistry determines their potency as vaccine nanocarriers in vitro and in vivo. Journal of Controlled Release. 225, pp. 205-216. https://doi.org/10.1016/j.jconrel.2016.01.030
AuthorsHassan, Hatem A.F.M., Smyth, L., Rubio, Noelia, Ratnasothy, Kulachelvy, Wang, Julie T.-W., Bansal, Sukhvinder S., Summers, Huw D., Diebold, Sandra S., Lombardi, Giovanna and Al-Jamal, Khuloud T.
Abstract

Carbon nanotubes (CNTs) have shown marked capabilities in enhancing antigen delivery to antigen presenting
cells. However, proper understanding of how altering the physical properties of CNTs may influence antigen uptake
by antigen presenting cells, such as dendritic cells (DCs), has not been established yet.Wehypothesized that
altering the physical properties of multi-walled CNTs (MWNTs)-antigen conjugates, e.g. length and surface
charge, can affect the internalization of MWNT-antigen by DCs, hence the induced immune response potency.
For this purpose, pristineMWNTs (p-MWNTs) were exposed to various chemical reactions to modify their physical
properties then conjugated to ovalbumin (OVA), a model antigen. The yieldedMWNTs-OVA conjugateswere
longMWNT-OVA (~386 nm), bearing net positive charge (5.8mV), or shortMWNTs-OVA (~122 nm) of increasing
negative charges (−23.4, −35.8 or −39 mV). Compared to the short MWNTs-OVA bearing high negative
charges, short MWNT-OVA with the lowest negative charge demonstrated better cellular uptake and OVAspecific
immune response both in vitro and in vivo. However, long positively-chargedMWNT-OVA showed limited
cellular uptake and OVA specific immune response in contrast to shortMWNT-OVA displaying the least negative
charge. We suggest that reduction in charge negativity of MWNT-antigen conjugate enhances cellular
uptake and thus the elicited immune response intensity. Nevertheless, length of MWNT-antigen conjugate
might also affect the cellular uptake and immune response potency; highlighting the importance of physical
properties as a consideration in designing a MWNT-based vaccine delivery system.

JournalJournal of Controlled Release
Journal citation225, pp. 205-216
ISSN01683659
Year2016
PublisherElsevier
Publisher's version
License
CC BY
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jconrel.2016.01.030
Publication dates
Print21 Jan 2016
Publication process dates
Deposited09 Feb 2016
Accepted18 Jan 2016
FunderBiotechnology and Biological Sciences Research Council
Worldwide Cancer Research
EU FP7-ITN Marie-Curie Network programme RADDEL
COST Action
YCCF
BHF Programme
Copyright information© 2016 The Authors. Open Access funded by Biotechnology and Biological Sciences Research Council under a Creative Commons License (CC-BY)
Permalink -

https://repository.uel.ac.uk/item/85297

Log in to edit

Download files

Publisher's version
Carbon nanotubes' surface chemistry determines their potency as.pdf
License: CC BY

  • 206
    total views
  • 298
    total downloads
  • 0
    views this month
  • 2
    downloads this month

Export as

Related outputs

Radiolabelling of Polyclonally Expanded Human Regulatory T Cells (Treg) with ⁸⁹Zr-oxine for Medium-Term In Vivo Cell Tracking
Jacob, J., Volpe, A., Peng, Q., Lechler, R. I., Smyth, L. A., Lombardi, G. and Fruhwirth, G. O. 2023. Radiolabelling of Polyclonally Expanded Human Regulatory T Cells (Treg) with ⁸⁹Zr-oxine for Medium-Term In Vivo Cell Tracking. Molecules. 28 (Art. 1482). https://doi.org/10.3390/molecules28031482
Inhibition of thrombin on endothelium enhances recruitment of regulatory T cells during IRI and when combined with adoptive Treg transfer, significantly protects against acute tissue injury and prolongs allograft survival
Peng, Q., Nowocin, A., Ratnasothy, K., Smith, R. A., Smyth, L., Lechler, R. I., Dorling, A. and Lombardi, G. 2023. Inhibition of thrombin on endothelium enhances recruitment of regulatory T cells during IRI and when combined with adoptive Treg transfer, significantly protects against acute tissue injury and prolongs allograft survival. Frontiers in Immunology. 13 (Art. 980462). https://doi.org/10.3389/fimmu.2022.980462
Editorial: Characterisation, functions and roles of antigen-specific regulatory T cells in health and disease
Tran,G. T., Verma, N. D., Smyth, L. and Hall, B. M. 2022. Editorial: Characterisation, functions and roles of antigen-specific regulatory T cells in health and disease. Frontiers in Immunology. 13. https://doi.org/10.3389/fimmu.2022.1022813
Nox2-deficient Tregs improve heart transplant outcomes via their increased graft recruitment and enhanced potency
Trevelin, S. C., Zampetaki, A., Sawyer, G., Ivetic, A., Brewer, A. C., Smyth, L. A., Marelli-Berg, F., Köchl, R., Lechler, R. I., Shah, A. M. and Lombardi, G. 2021. Nox2-deficient Tregs improve heart transplant outcomes via their increased graft recruitment and enhanced potency. JCI Insight. 6 (Art. e149301). https://doi.org/10.1172/jci.insight.149301
Spatiotemporal in vivo tracking of polyclonal human regulatory T cells reveals a role for innate immune cells in Treg transplant recruitment
Jacob, J., Nadkarni, S., Volpe, A., Peng, Q., Tung, S. L., Hannen, R. F., Mohseni, Y. R., Scotta, C., Marelli-Berg, F. M., Lechler, R. I., Smyth, L., Fruhwirth, G. O. and Lombardi, G. 2020. Spatiotemporal in vivo tracking of polyclonal human regulatory T cells reveals a role for innate immune cells in Treg transplant recruitment. Molecular Therapy - Methods and Clinical Development. 20, pp. 324-336. https://doi.org/10.1016/j.omtm.2020.12.003
B lymphocytes contribute to indirect pathway T cell sensitisation via acquisition of extracellular vesicles
Becker, P. D., Ratnasothy, K., Sen, M., Peng, Q., Romano, M., Bazoer, J., Suvitra, E., Stout, A., Hylton, S. G., Dorling, A., Lechler, R. I., Smyth, L. and Lombardi, G. 2020. B lymphocytes contribute to indirect pathway T cell sensitisation via acquisition of extracellular vesicles. American Journal of Transplantation. 21 (4), pp. 1415-1426. https://doi.org/10.1111/ajt.16088
Regulatory T Cell Extracellular Vesicles Modify T-Effector Cell Cytokine Production and Protect Against Human Skin Allograft Damage
Tung, S. L., Fanelli, G., Matthews, R. I., Bazoer, J., Letizia, M., Vizcay-Barrena, G., Faruqu, F. N., Philippeos, C., Hannen, R., Al-Jamal, K. T., Smyth, L. and Lombardi, G. 2020. Regulatory T Cell Extracellular Vesicles Modify T-Effector Cell Cytokine Production and Protect Against Human Skin Allograft Damage. Frontiers in Cell and Developmental Biology. 8 (Art. 317). https://doi.org/10.3389/fcell.2020.00317
Application of carbon nanotubes in cancer vaccines: Achievements, challenges and chances
Hassan, Hatem A.F.M., Diebold, Sandra S., Smyth, L., Walters, Adam A., Lombardi, Giovanna and Al-Jamal, Khuloud T. 2019. Application of carbon nanotubes in cancer vaccines: Achievements, challenges and chances. Journal of Controlled Release. 297, pp. 79-90. https://doi.org/10.1016/j.jconrel.2019.01.017
Protease Activated Receptor 4 as a Novel Modulator of Regulatory T Cell Function
Peng, Q., Ratnasothy, K., Boardman, D. A., Jacob, J., Tung, S. T., McCluskey, D., Smyth, L., Lechler, R. I., Dorling, A. and Lombardi, G. 2019. Protease Activated Receptor 4 as a Novel Modulator of Regulatory T Cell Function. Frontiers in Immunology. 10 (Art. 1311). https://doi.org/10.3389/fimmu.2019.01311
Membrane radiolabelling of exosomes for comparative biodistribution analysis in immunocompetent and immunodeficient mice – a novel and universal approach
Faruqu, Farid N., Wang, Julie Tzu-Wen, Xu, Lizhou, McNickle, Luke, Chong, Eden Ming-Yiu, Walters, Adam, Gurney, Mark, Clayton, Aled, Smyth, L., Hider, Robert, Sosabowski, Jane and Al-Jamal, Khuloud T. 2019. Membrane radiolabelling of exosomes for comparative biodistribution analysis in immunocompetent and immunodeficient mice – a novel and universal approach. Theranostics. 9 (6), pp. 1666-1682. https://doi.org/10.7150/thno.27891
Nox2 in regulatory T cells promotes angiotensin II–induced cardiovascular remodeling
Emmerson, Amber, Trevelin, Silvia Cellone, Mongue-Din, Heloise, Becker, Pablo D., Ortiz, Carla, Smyth, L., Peng, Qi, Elgueta, Raul, Sawyer, Greta, Ivetic, Aleksandar, Lechler, Robert I., Lombardi, Giovanna and Shah, Ajay M. 2018. Nox2 in regulatory T cells promotes angiotensin II–induced cardiovascular remodeling. Journal of Clinical Investigation. 128 (7), pp. 3088-3101. https://doi.org/10.1172/JCI97490
Regulatory T cell-derived extracellular vesicles modify dendritic cell function
Tung, Sim L., Boardman, Dominic A., Sen, Monica, Letizia, Marilena, Peng, Qi, Cianci, Nicole, Dioni, Laura, Carlin, Leo M., Lechler, Robert, Bollati, Valentina, Lombardi, Giovanna and Smyth, L. 2018. Regulatory T cell-derived extracellular vesicles modify dendritic cell function. Scientific Reports. 8, p. 6065. https://doi.org/10.1038/s41598-018-24531-8
Galectin-1 is required for the regulatory function of B cells
Alhabbab, R., Blair, P., Smyth, L., Ratnasothy, K., Peng, Q., Moreau, A., Lechler, R., Elgueta, R. and Lombardi, G. 2018. Galectin-1 is required for the regulatory function of B cells. Scientific Reports. 8 (2725). https://doi.org/10.1038/s41598-018-19965-z
Constitutive expression of the anti-apoptotic Bcl-2 family member A1 in murine endothelial cells leads to transplant tolerance
Smyth, L., Meader, Lucy, Xiao, Fang, Woodward, Martin, Brady, Hugh J., Lechler, Robert and Lombardi, Giovanna 2017. Constitutive expression of the anti-apoptotic Bcl-2 family member A1 in murine endothelial cells leads to transplant tolerance. Clinical and Experimental Immunology. 188 (2), pp. 219-225. https://doi.org/10.1111/cei.12931
Dual stimulation of antigen presenting cells using carbon nanotube-based vaccine delivery system for cancer immunotherapy
Hassan, Hatem A.F.M., Smyth, L., Wang, Julie T.-W., Costa, Pedro M., Ratnasothy, Kulachelvy, Diebold, Sandra S., Lombardi, Giovanna and Al-Jamal, Khuloud T. 2016. Dual stimulation of antigen presenting cells using carbon nanotube-based vaccine delivery system for cancer immunotherapy. Biomaterials. 104, pp. 310-322. https://doi.org/10.1016/j.biomaterials.2016.07.005
Regulatory T Cell-Derived Exosomes: Possible Therapeutic and Diagnostic Tools in Transplantation
Agarwal, Akansha, Fanelli, Giorgia, Letizia, Marilena, Tung, Sim Lai, Boardman, Dominic, Lechler, Robert, Lombardi, Giovanna and Smyth, L. 2014. Regulatory T Cell-Derived Exosomes: Possible Therapeutic and Diagnostic Tools in Transplantation. Frontiers in Immunology. 5.
UK–Russia Researcher Links Workshop: extracellular vesicles – mechanisms of biogenesis and roles in disease pathogenesis, M.V. Lomonosov Moscow State University, Moscow, Russia, 1–5 March 2015
Kapustin, Alexander N., Kalinina, Natalia, Lopatina, Tatiana, Davidson, Sean M., Iraci, Nunzio, Tamkovich, Svetlana, Smyth, L., Ter-Ovanesyan, Dmitry, Evtushenko, Evgeniy G., Savelieva, Olga, Bertazzo, Sergio, Aushev, Vassiliy, Dragovic, Rebecca, Gracia, Tannia, Heck, Margarete, Parfyonova, Yelena V., Shanahan, Catherine M. and Tkachuk, Vsevolod 2015. UK–Russia Researcher Links Workshop: extracellular vesicles – mechanisms of biogenesis and roles in disease pathogenesis, M.V. Lomonosov Moscow State University, Moscow, Russia, 1–5 March 2015. Journal of Extracellular Vesicles. 4 (1), p. 28094. https://doi.org/10.3402/jev.v4.28094
Diversity of gut microflora is required for the generation of B cell with regulatory properties in a skin graft model
Alhabbab, R., Blair, P., Elgueta, R., Stolarczyk, E., Marks, E., Becker, P. D., Ratnasothy, K., Smyth, L., Safinia, N., Sharif-Paghaleh, E., O’Connell, S., Noelle, R. J., Lord, G. M., Howard, J. K., Spencer, J., Lechler, R. I. and Lombardi, G. 2015. Diversity of gut microflora is required for the generation of B cell with regulatory properties in a skin graft model. Scientific Reports. 5, p. 11554.
Continuous acquisition of MHC:peptide complexes by recipient cells contributes to the generation of anti-graft CD8+T cell immunity
Smyth, L., Lechler, Robert I and Lombardi, Giovanna 2016. Continuous acquisition of MHC:peptide complexes by recipient cells contributes to the generation of anti-graft CD8+T cell immunity. American Journal of Transplantation. 17 (1), pp. 60-68. https://doi.org/10.1111/ajt.13996
What Is Direct Allorecognition?
Boardman, Dominic A., Jacob, Jacinta, Smyth, L., Lombardi, Giovanna and Lechler, Robert I. 2016. What Is Direct Allorecognition? Current Transplantation Reports. 3 (4), pp. 275-283. https://doi.org/10.1007/s40472-016-0115-8
Treg therapy in transplantation a general overview
Romano, Marco, Tung, Sim Lai, Smyth, L. and Lombardi, Giovanna 2016. Treg therapy in transplantation a general overview. Transplant International. 30 (8), pp. 745-753. https://doi.org/10.1111/tri.12909
Expression of a chimeric antigen receptor specific for donor HLA class I enhances the potency of human regulatory T cells in preventing human skin transplant rejection
Boardman, Dominic A., Philippeos, Christina, Fruhwirth, Gilbert O., Ibrahim, Mohammad A. A., Hannen, Rosalind F., Cooper, Dianne, Marelli-Berg, Federica M., Watt, Fiona M., Lechler, Robert I., Maher, John, Smyth, L. and Lombardi, Giovanna 2016. Expression of a chimeric antigen receptor specific for donor HLA class I enhances the potency of human regulatory T cells in preventing human skin transplant rejection. American Journal of Transplantation. 17 (4), pp. 931-943. https://doi.org/10.1111/ajt.14185