Spotsizer: High-throughput quantitative analysis of microbial growth

Article

Bischof, Leanne, Převorovský, Martin, Rallis, C., Jeffares, Daniel C., Arzhaeva, Yulia and Bähler, Jürg 2016. Spotsizer: High-throughput quantitative analysis of microbial growth. BioTechniques. 61 (4), pp. 191-201. https://doi.org/10.2144/000114459
AuthorsBischof, Leanne, Převorovský, Martin, Rallis, C., Jeffares, Daniel C., Arzhaeva, Yulia and Bähler, Jürg
Abstract

Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.

JournalBioTechniques
Journal citation61 (4), pp. 191-201
ISSN0736-6205
Year2016
PublisherEaton Publishing
Publisher's version
License
File Access Level
Anyone
Digital Object Identifier (DOI)https://doi.org/10.2144/000114459
Web address (URL)https://doi.org/10.2144/000114459
Publication dates
Print01 Oct 2016
Online16 Mar 2018
Publication process dates
Deposited07 Feb 2018
Accepted02 Oct 2016
FunderCzech Science Foundation
Charles University in Prague
Commonwealth Scientific and Industrial Research Organisation of Australia
Biotechnology and Biological Sciences Research Council
Wellcome Trust
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