Spotsizer: High-throughput quantitative analysis of microbial growth
Bischof, Leanne, Převorovský, Martin, Rallis, C., Jeffares, Daniel C., Arzhaeva, Yulia and Bähler, Jürg 2016. Spotsizer: High-throughput quantitative analysis of microbial growth. BioTechniques. 61 (4), pp. 191-201.
|Authors||Bischof, Leanne, Převorovský, Martin, Rallis, C., Jeffares, Daniel C., Arzhaeva, Yulia and Bähler, Jürg|
Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.
|Journal citation||61 (4), pp. 191-201|
|Digital Object Identifier (DOI)||doi:10.2144/000114459|
|Web address (URL)||https://doi.org/10.2144/000114459|
|01 Oct 2016|
|Publication process dates|
|Deposited||07 Feb 2018|
|Funder||Czech Science Foundation|
|Charles University in Prague|
|Commonwealth Scientific and Industrial Research Organisation of Australia|
|Biotechnology and Biological Sciences Research Council|
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