Cell-autonomous integrin control of Wnt and Notch signalling during somitogenesis

Article

Rallis, C., Pinchin, S. M. and Ish-Horowicz, D. 2010. Cell-autonomous integrin control of Wnt and Notch signalling during somitogenesis. Development. 137 (21), pp. 3591-3601. https://doi.org/10.1242/dev.050070
AuthorsRallis, C., Pinchin, S. M. and Ish-Horowicz, D.
Abstract

Integrins act at signalling crossroads, and their interactions with other signal transduction pathways are key to the regulation of normal and pathological cell cytoarchitecture and behaviour. Here, we describe a signalling cascade that acts during the formation of the defining segmental features of the vertebrate body – the somites – in which β1-integrin activity regulates epithelialisation by controlling downstream Wnt and Notch activity crucial for somite border formation. Using in vivo transcriptional inhibition in the developing chick embryo, we show that β1-integrin in the anterior presomitic mesoderm activates canonical Wnt signalling in a cell-autonomous, `outside-inside' manner. Signalling is mediated by integrin-linked kinase (ILK), leading to modulation of glycogen synthase kinase 3β (GSK3β) phosphorylation, and activates Notch signalling in the anterior presomitic mesoderm. The two signalling pathways then cooperate to promote somite formation via cMESO1/Mesp2. Our results show that β1-integrin can regulate cell shape and tissue morphogenesis indirectly, by regulation of downstream signalling cascades.

JournalDevelopment
Journal citation137 (21), pp. 3591-3601
ISSN0950-1991
Year2010
PublisherThe Company of Biologists Ltd
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Digital Object Identifier (DOI)https://doi.org/10.1242/dev.050070
Web address (URL)https://doi.org/10.1242/dev.050070
Publication dates
Online28 Sep 2010
Publication process dates
Deposited13 Feb 2018
Accepted16 Aug 2010
Accepted16 Aug 2010
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