Identification of nuclear genes affecting 2-Deoxyglucose resistance in Schizosaccharomyces pombe

Article


Vishwanatha, Akshay, Rallis, C., Bevkal Subramanyaswamy, Shubha, D'Souza, Cletus Joseph Michael, Bähler, Jürg, Schweingruber, Martin Ernst and Dawes, Ian 2016. Identification of nuclear genes affecting 2-Deoxyglucose resistance in Schizosaccharomyces pombe. FEMS Yeast Research. 16 (6), p. fow061. https://doi.org/10.1093/femsyr/fow061
AuthorsVishwanatha, Akshay, Rallis, C., Bevkal Subramanyaswamy, Shubha, D'Souza, Cletus Joseph Michael, Bähler, Jürg, Schweingruber, Martin Ernst and Dawes, Ian
Abstract

2-Deoxyglucose (2-DG) is a toxic glucose analog. To identify genes involved in 2-DG toxicity in Schizosaccharomyces pombe, we
screened a wild-type overexpression library for genes which render cells 2-DG resistant. A gene we termed odr1, encoding
an uncharacterized hydrolase, led to strong resistance and altered invertase expression when overexpressed. We speculate
that Odr1 neutralizes the toxic form of 2-DG, similar to the Saccharomyces cerevisiae Dog1 and Dog2 phosphatases which
dephosphorylate 2-DG-6-phosphate synthesized by hexokinase. In a complementary approach, we screened a haploid
deletion library to identify 2-DG-resistant mutants. This screen identified the genes snf5, ypa1, pas1 and pho7. In liquid
medium, deletions of these genes conferred 2-DG resistance preferentially under glucose-repressed conditions. The
deletion mutants expressed invertase activity more constitutively than the control strain, indicating defects in the control
of glucose repression. No S. cerevisiae orthologs of the pho7 gene is known, and no 2-DG resistance has been reported for any
of the deletion mutants of the other genes identified here. Moreover, 2-DG leads to derepressed invertase activity in S.
pombe, while in S. cerevisiae it becomes repressed. Taken together, these findings suggest that mechanisms involved in 2-DG
resistance differ between budding and fission yeasts.

JournalFEMS Yeast Research
Journal citation16 (6), p. fow061
ISSN1567-1364
Year2016
PublisherOxford University Press for Federation of European Microbiological Societies
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Digital Object Identifier (DOI)https://doi.org/10.1093/femsyr/fow061
Web address (URL)https://doi.org/10.1093/femsyr/fow061
Publication dates
Print31 Jul 2016
Publication process dates
Deposited07 Feb 2018
Accepted25 Jul 2016
Accepted25 Jul 2016
Copyright information© 2016 The authors
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