Cell-based screens and phenomics with fission yeast

Article


Rallis, C. and Bähler, Jürg 2015. Cell-based screens and phenomics with fission yeast. Critical Reviews in Biochemistry and Molecular Biology. 51 (2), pp. 86-95.
AuthorsRallis, C. and Bähler, Jürg
Abstract

Next-generation sequencing approaches have considerably advanced our understanding of genome function and regulation. However, the knowledge of gene function and complex cellular processes remains a challenge and bottleneck in biological research. Phenomics is a rapidly emerging area, which seeks to rigorously characterize all phenotypes associated with genes or gene variants. Such high-throughput phenotyping under different conditions can be a potent approach toward gene function. The fission yeast Schizosaccharomyces pombe (S. pombe) is a proven eukaryotic model organism that is increasingly used for genomewide screens and phenomic assays. In this review, we highlight current large-scale, cell-based approaches used with S. pombe, including computational colony-growth measurements, genetic interaction screens, parallel profiling using barcodes, microscopy-based cell profiling, metabolomic methods and transposon mutagenesis. These diverse methods are starting to offer rich insights into the relationship between genotypes and phenotypes.

JournalCritical Reviews in Biochemistry and Molecular Biology
Journal citation51 (2), pp. 86-95
ISSN1549-7798
1040-9238
Year2015
PublisherTaylor & Francis
Digital Object Identifier (DOI)doi:10.3109/10409238.2015.1103205
Publication dates
Print02 Nov 2015
Publication process dates
Deposited27 Feb 2017
Copyright information© 2015 Taylor & Francis
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