Cell-based screens and phenomics with fission yeast
Rallis, C. and Bähler, Jürg 2015. Cell-based screens and phenomics with fission yeast. Critical Reviews in Biochemistry and Molecular Biology. 51 (2), pp. 86-95.
|Authors||Rallis, C. and Bähler, Jürg|
Next-generation sequencing approaches have considerably advanced our understanding of genome function and regulation. However, the knowledge of gene function and complex cellular processes remains a challenge and bottleneck in biological research. Phenomics is a rapidly emerging area, which seeks to rigorously characterize all phenotypes associated with genes or gene variants. Such high-throughput phenotyping under different conditions can be a potent approach toward gene function. The fission yeast Schizosaccharomyces pombe (S. pombe) is a proven eukaryotic model organism that is increasingly used for genomewide screens and phenomic assays. In this review, we highlight current large-scale, cell-based approaches used with S. pombe, including computational colony-growth measurements, genetic interaction screens, parallel profiling using barcodes, microscopy-based cell profiling, metabolomic methods and transposon mutagenesis. These diverse methods are starting to offer rich insights into the relationship between genotypes and phenotypes.
|Journal||Critical Reviews in Biochemistry and Molecular Biology|
|Journal citation||51 (2), pp. 86-95|
|Publisher||Taylor & Francis|
|Digital Object Identifier (DOI)||doi:10.3109/10409238.2015.1103205|
|02 Nov 2015|
|Publication process dates|
|Deposited||27 Feb 2017|
|Copyright information||© 2015 Taylor & Francis|
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