A central role for TOR signalling in a yeast model for juvenile CLN3 disease

Article

Bond, Michael, Brown, Rachel, Rallis, C., Bahler, Jurg and Mole, Sara 2015. A central role for TOR signalling in a yeast model for juvenile CLN3 disease. Microbial Cell. 2 (12), pp. 466-480.
AuthorsBond, Michael, Brown, Rachel, Rallis, C., Bahler, Jurg and Mole, Sara
Abstract

Yeasts provide an excellent genetically tractable eukaryotic system for investigating the function of genes in their biological context, and are especially relevant for those conserved genes that cause disease. We study the role of btn1, the orthologue of a human gene that underlies an early onset neurodegenerative disease (juvenile CLN3 disease, neuronal ceroid lipofuscinosis (NCLs) or Batten disease) in the fission yeast Schizosaccharomyces pombe. A global screen for genetic interactions with btn1 highlighted a conserved key signalling hub in which multiple components functionally relate to this conserved disease gene. This signalling hub includes two major mitogen-activated protein kinase (MAPK) cascades, and centers on the Tor kinase complexes TORC1 and TORC2. We confirmed that yeast cells modelling CLN3 disease exhibit features consistent with dysfunction in the TORC pathways, and showed that modulating TORC function leads to a comprehensive rescue of defects in this yeast disease model. The same pathways may be novel targets in the development of therapies for the NCLs and related diseases.

JournalMicrobial Cell
Journal citation2 (12), pp. 466-480
ISSN23112638
Year2015
PublisherShared Science Publishers
Publisher's version
License
CC BY
Web address (URL)http://dx.doi.org/10.15698/mic2015.12.241
Publication dates
Print11 Nov 2015
Publication process dates
Deposited04 Feb 2016
Accepted14 Oct 2015
FunderEuropean Community Seventh Framework Programme
Batten Disease Family Association, UK
Children's Batten Disease Foundation, USA
Copyright information© 2015 Bond et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
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