Bartonella vinsonii sub. arupensis infection in animals of veterinary importance, ticks and biopsy samples

Testing for vector-borne pathogens in livestock is largely reliant upon blood and tissue. The role of biopsy samples remains poorly explored for detecting tick-borne bacteria in animals.

In a 2-year survey, animals of veterinary importance from farms throughout the Northern part of Greece were routinely checked for the presence of biopsy samples. Where detected, either a portion or biopsy was collected together with whole blood samples and any ticks at the site of the biopsy sample. Molecular testing was carried out by real-time PCR targeting the ITS gene of Bartonella species.

A total 68 samples [28 blood samples, 28 biopsy samples and 12 ticks (9 Rhipicephalus bursa and 3 R. turanicus)] were collected from goats (64 samples) and bovine (4 samples).

Eight (11.8%) of the 68 samples were positive for Bartonella species. Of the biopsy sample and whole blood samples, four (14.3%) of each type were positive for Bartonella species. None of the ticks was tested positive for Bartonella species. All pairs of positive biopsy samples/whole blood samples originated from the same animals. Positive samples were identified as B. vinsonii sub. arupensis.

Although many more samples from a much wider spectrum of animal species is required before concluding upon the merit of biopsy samples on the study of tick-borne diseases, the significance of our finding warrants further study, both for clinical consequences in small ruminants and for those humans farming infected animals.