Bartonella vinsonii sub. arupensis infection in animals of veterinary importance, ticks and biopsy samples
Chochlakis, D., Cutler, S., Giadinis, N. D. and Psaroulaki, A. 2020. Bartonella vinsonii sub. arupensis infection in animals of veterinary importance, ticks and biopsy samples. New Microbes and New Infections. 34 (Art. 100652). https://doi.org/10.1016/j.nmni.2020.100652
|Authors||Chochlakis, D., Cutler, S., Giadinis, N. D. and Psaroulaki, A.|
Testing for vector-borne pathogens in livestock is largely reliant upon blood and tissue. The role of biopsy samples remains poorly explored for detecting tick-borne bacteria in animals.
In a 2-year survey, animals of veterinary importance from farms throughout the Northern part of Greece were routinely checked for the presence of biopsy samples. Where detected, either a portion or biopsy was collected together with whole blood samples and any ticks at the site of the biopsy sample. Molecular testing was carried out by real-time PCR targeting the ITS gene of Bartonella species.
A total 68 samples [28 blood samples, 28 biopsy samples and 12 ticks (9 Rhipicephalus bursa and 3 R. turanicus)] were collected from goats (64 samples) and bovine (4 samples).
Eight (11.8%) of the 68 samples were positive for Bartonella species. Of the biopsy sample and whole blood samples, four (14.3%) of each type were positive for Bartonella species. None of the ticks was tested positive for Bartonella species. All pairs of positive biopsy samples/whole blood samples originated from the same animals. Positive samples were identified as B. vinsonii sub. arupensis.
Although many more samples from a much wider spectrum of animal species is required before concluding upon the merit of biopsy samples on the study of tick-borne diseases, the significance of our finding warrants further study, both for clinical consequences in small ruminants and for those humans farming infected animals.
|Journal||New Microbes and New Infections|
|Journal citation||34 (Art. 100652)|
|Accepted author manuscript|
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|Digital Object Identifier (DOI)||https://doi.org/10.1016/j.nmni.2020.100652|
|Web address (URL)||https://doi.org/10.1016/j.nmni.2020.100652|
|Online||16 Jan 2020|
|Publication process dates|
|Accepted||03 Jan 2020|
|Deposited||20 Jan 2020|
|Copyright holder||© 2020 Elsevier|
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