The Role of G Protein In Alcohol Related Behaviours Using Drosophila melanogaster As A Model

Conference poster

Aleyakpo, B., Umokoro, Oghenetega, Corcoran, O. and Casalotti, S. 2015. The Role of G Protein In Alcohol Related Behaviours Using Drosophila melanogaster As A Model. British Pharmacological Society Annual meeting. London Dec 2015 PA2 online British Pharmacological Society. pp. 307-307
AuthorsAleyakpo, B., Umokoro, Oghenetega, Corcoran, O. and Casalotti, S.
TypeConference poster
Abstract

Ethanol, classified as a drug, affects the central nervous system, and its consumption has been linked to the development of several behaviours including tolerance and dependence. Alcohol tolerance is defined as the need for higher doses of alcohol to induce the same changes observed in the initial exposure or where repetitive exposures of the same alcohol dose induce a lower response. Ethanol has been shown to interact with numerous targets and ultimately influence both short and long term adaptation at the cellular and molecular level in brain [1]. These adaptation processes are likely to involve signalling molecules: our work has focussed on G proteins gene expression. Using both wild type and several mutant fruit fly (Drosophila melanogaster) as a model for behaviour and molecular studies, we observed significant increases in sedation time (ST50) in response to alcohol (P<0.001) Fig.A. We also observed a consistent and significant decrease of Gq protein mRNA expression in Drosophila dUNC and DopR2 mutants chronically exposed to alcohol (*P<0.05). Fig B.
Method: Six male flies were observed in drosophila polystyrene 25 x 95mm transparent vial in between cotton plugs. To the top plug, 500uL of 100% ethanol was added. Time till 50% of the flies were sedated was recorded on each day following the schedule. Fig. C (n=4-6). Using RT-PCR, we also quantified G protein mRNA expression levels one hour post initial 30 minutes of ethanol expression on day 1 and day 3 relative to expression in naïve flies.(n=2)

[A] Increase in sedation time indicative of tolerance in different mutant lines and wild type flies. Six male flies were used in each experiment and (n= 4-6. ***P<0.001 unpaired t tests). [B] RT-PCR results showing significant reduction in Gq mRNA in flies chronically exposed to alcohol. (n=2. *P<0.05) [C] Alcohol exposure schedule.

(1) Kaun K.R., R. Azanchi, Z. Maung, J. Hirsh, U. Heberlein. (2011). A Drosophila model for alcohol reward. Nature Neuroscience. 14 (5), 612–619.

Keywordssubstance misuse; addiction; fruit flies; gene expression; qRT-PCR
Year2015
ConferenceBritish Pharmacological Society Annual meeting
PublisherBritish Pharmacological Society
Publisher's version
License
CC BY
Publication dates
Print2015
Publication process dates
Deposited14 Jul 2016
Web address (URL)http://www.pa2online.org/abstracts/vol13issue3abst307p.pdf
Place of publicationPA2 online
Page range307-307
Permalink -

https://repository.uel.ac.uk/item/8579w

Log in to edit

Download files

Publisher's version
Ben Gprotein vol13issue3abst307p.pdf
License: CC BY

  • 266
    total views
  • 76
    total downloads
  • 4
    views this month
  • 2
    downloads this month

Export as

Related outputs

Prebiotics may alter bile salt hydrolase activity: Possible implications for cholesterol metabolism
Adebola, O., Corcoran, O. and Morgan, W. 2020. Prebiotics may alter bile salt hydrolase activity: Possible implications for cholesterol metabolism. PharmaNutrition. 12 (Art. 100182). https://doi.org/10.1016/j.phanu.2020.100182
G-protein αq gene expression plays a role in alcohol tolerance in Drosophila melanogaster
Aleyakpo, B., Umukoro, O., Kavlie, R., Ranson, D., Thompsett, A., Corcoran, O. and Casalotti, S. 2019. G-protein αq gene expression plays a role in alcohol tolerance in Drosophila melanogaster. Brain and Neuroscience Advances. 3. https://doi.org/10.1177/2398212819883081
Jigsaw Recovery: The Spatio-temporalities of Alcohol Abuse and Recovery in a Non-interventionist, Peer-led Service
Kougiali, Z., Soar, K., Pytlik, A., Brown, J., Wozniak, K., Nsumba, F., Wilkins, T., Oliva, E., Osunsanya, S., Clarke, C., Casalotti, S. and Pendry, B. 2019. Jigsaw Recovery: The Spatio-temporalities of Alcohol Abuse and Recovery in a Non-interventionist, Peer-led Service. Alcoholism Treatment Quarterly. 38 (2), pp. 165-183. https://doi.org/10.1080/07347324.2019.1686960
From Scutellaria barbata to BZL101 in Cancer Patients: Phytochemistry, Pharmacology, and Clinical Evidence
Gao, J., Yin, W. and Corcoran, O. 2019. From Scutellaria barbata to BZL101 in Cancer Patients: Phytochemistry, Pharmacology, and Clinical Evidence. Natural Product Communications. 14 (10). https://doi.org/10.1177/1934578X19880645
The Role of Oestrogen Receptor Beta (ERβ) in the Aetiology and Treatment of Type 2 Diabetes Mellitus
Ofosu, W. A., Mohamed, D., Corcoran, O. and Ojo, O. 2019. The Role of Oestrogen Receptor Beta (ERβ) in the Aetiology and Treatment of Type 2 Diabetes Mellitus. Current Diabetes Reviews. 15 (2), pp. 100-104. https://doi.org/10.2174/1573399814666180119141836
Pharmacological targeting of the GABAʙ receptor alters Drosophila's behavioural responses to alcohol
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2019. Pharmacological targeting of the GABAʙ receptor alters Drosophila's behavioural responses to alcohol. Addiction Biology. 25 (Art. e12725). https://doi.org/10.1111/adb.12725
Role of GABAB receptors in ethanol induced behaviours
Ranson, D., Ayoub, S. and Casalotti, S. 2017. Role of GABAB receptors in ethanol induced behaviours. pA2 Online: Proceedings of the British Pharmacological Society. 18 (1).
Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster. 11th FENS Forum of Neuroscience. Berlin, Germany 07 - 11 Jul 2018
Naltrexone Reverses Ethanol Preference and Protein Kinase C Activation in Drosophila melanogaster
Koyyada, Rajeswari, Latchooman, Nilesh, Jonaitis, Julius, Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Naltrexone Reverses Ethanol Preference and Protein Kinase C Activation in Drosophila melanogaster. Frontiers in Physiology. 9 (175), pp. 1-7. https://doi.org/10.3389/fphys.2018.00175
Role of gamma-aminobutyric acid-B receptors in ethanol induced behaviours
Ranson, D., Ayoub, S. and Casalotti, S. 2017. Role of gamma-aminobutyric acid-B receptors in ethanol induced behaviours. Society for the Study of Addiction Conference. Newcastle, UK 08 - 10 Nov 2017
Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects (Study Data)
Dawkins, L., Kimber, C., Doig, Mira, Feyerabend, Colin and Corcoran, O. 2018. Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects (Study Data). The University of East London. https://doi.org/10.15123/DATA.00000223
Identification and preliminary structure-activity relationship studies of novel pyridyl sulfonamides as potential Chagas disease therapeutic agents
Peres, Raiza Brandão, Ullah, Asma Inam, de Almeida Fiuza, Ludmila Ferreira, Silva, Patricia Bernardino, Batista, Marcos M., Corcoran, O., Reddy, T. and de Nazaré Correia Soeiro, Maria 2018. Identification and preliminary structure-activity relationship studies of novel pyridyl sulfonamides as potential Chagas disease therapeutic agents. Bioorganic & Medicinal Chemistry Letters. 28 (11), pp. 2018-2022. https://doi.org/10.1016/j.bmcl.2018.04.064
Compensatory Puffing With Lower Nicotine Concentration E-liquids Increases Carbonyl Exposure in E-cigarette Aerosols
Kośmider, Leon, Kimber, C., Kurek, Jolanta, Corcoran, O. and Dawkins, Lynne E. 2018. Compensatory Puffing With Lower Nicotine Concentration E-liquids Increases Carbonyl Exposure in E-cigarette Aerosols. Nicotine & Tobacco Research. 20 (8), pp. 998-1003. https://doi.org/10.1093/ntr/ntx162
Synbiotics: the impact of potential prebiotics inulin, lactulose and lactobionic acid on the survival and growth of lactobacilli probiotics
Adebola, Oluwakemi Obasola, Corcoran, O. and Morgan, Winston A. 2014. Synbiotics: the impact of potential prebiotics inulin, lactulose and lactobionic acid on the survival and growth of lactobacilli probiotics. Journal of Functional Foods. 10, pp. 75-84. https://doi.org/10.1016/j.jff.2014.05.010
Silymarin content in Silybum marianum extracts as a biomarker for the quality of commercial tinctures
Pendry, B., Kemp, Virginia, Hughes, Michael J., Freeman, Julia, Nuhu, Hanny K., Sanchez-Medina, Alberto, Corcoran, O. and Galante, E. 2017. Silymarin content in Silybum marianum extracts as a biomarker for the quality of commercial tinctures. Journal of Herbal Medicine. 10, pp. 31-36. https://doi.org/10.1016/j.hermed.2017.07.001
Ethanol-induced G-protein subunit expression changes in D2 receptor deficient Drosophila
Umokoro, Oghenetega, Corcoran, O., Thompsett, Andrew and Casalotti, S. 2015. Ethanol-induced G-protein subunit expression changes in D2 receptor deficient Drosophila. British Neuroscience Association Festival 2015. Edinburgh, UK 12 - 15 Apr 2015 British Neuroscience Association. pp. 297-297
Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects
Dawkins, Lynne E., Kimber, Catherine F., Doig, Mira, Feyerabend, Colin and Corcoran, O. 2016. Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects. Psychopharmacology. 233 (15), pp. 2933-2941. https://doi.org/10.1007/s00213-016-4338-2
Hit Discovery from Natural Products in Pharmaceutical R&D
Corcoran, O. 2015. Hit Discovery from Natural Products in Pharmaceutical R&D. Encyclopedia of Magnetic Resonance. 4 (2), pp. 455-462. https://doi.org/10.1002/9780470034590.emrstm1432
Salvinorin A content in legal high products of Salvia divinorum sold in Mexico
Hernández-Bello, Rafael, García-Rodríguez, Rosa Virginia, García-Sosa, Karlina, Peña-Rodríguez, Luis Manuel, Vázquez-Hernández, Maribel, Ramos-Morales, Fernando Rafael, Corcoran, O. and Alberto, Sánchez-Medina 2015. Salvinorin A content in legal high products of Salvia divinorum sold in Mexico. Forensic Science International. 249 (April), pp. 197-201. https://doi.org/10.1016/j.forsciint.2015.01.038
Acute electronic cigarette use: nicotine delivery and subjective effects in regular users
Dawkins, L. and Corcoran, O. 2014. Acute electronic cigarette use: nicotine delivery and subjective effects in regular users. Psychopharmacology. 231 (2), pp. 401-407. https://doi.org/10.1007/s00213-013-3249-8
Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts
Osei-Djarbeng, Samuel N., Cutler, S., Cutler, Ronald R. and Corcoran, O. 2014. Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts. European Journal of Medicinal Plants. 4 (7), pp. 835-843. https://doi.org/10.9734/EJMP/2014/9316
Ethanol-induced changes in G-proteins gene expression
Casalotti, S. 2013. Ethanol-induced changes in G-proteins gene expression. UEL Research and Knowledge Exchange Conference 2013. University of East London, London 26 Jun 2013 London University of East London.
Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells
Adebola, O., Corcoran, O. and Morgan, W. 2013. Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells. Food Research International. 52 (1), pp. 269-274. https://doi.org/10.1016/j.foodres.2013.03.024
Anglo-Saxon pharmacopoeia revisited: a potential treasure in drug discovery
Watkins, Frances, Pendry, B., Corcoran, O. and Sanchez-Medina, Alberto 2011. Anglo-Saxon pharmacopoeia revisited: a potential treasure in drug discovery. Drug Discovery Today. 16 (23-24), pp. 1069-1075.
β3-integrin is required for differentiation in OC-2 cells derived from mammalian embryonic inner ear
Brunetta, Ivan, Casalotti, S., Hart, Ian R, Forge, Andrew and Reynolds, Louise E 2012. β3-integrin is required for differentiation in OC-2 cells derived from mammalian embryonic inner ear. BMC Cell Biology. 13 (1), p. 5.
Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing formulations in 10th century England
Watkins, F., Pendry, B., Sanchez-Medina, A. and Corcoran, O. 2012. Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing formulations in 10th century England. Journal of Ethnopharmacology. 144 (2), pp. 408-415. https://doi.org/10.1016/j.jep.2012.09.031
The metabolic profiling of exercise intervention: exercise metabolomics
Lyons, A., Corcoran, O., Culpan, J. and Carpenter, R. 2012. The metabolic profiling of exercise intervention: exercise metabolomics. Biomedical Basis of Elite Performance 2012. London, United Kingdom 19 - 21 Mar 2012 The Physiological Society.
Antimicrobial and anti-quorum sensing activities of some Ghanaian medicinal plants
Osei-Djarbeng, S., Corcoran, O., Cutler, S. and Cutler, R. R. 2012. Antimicrobial and anti-quorum sensing activities of some Ghanaian medicinal plants. International Journal of Indigenous Medicinal Plants. 28 (10), pp. 221-226.
The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells
Gao, J., Morgan, W., Sanchez-Medina, A. and Corcoran, O. 2011. The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells. Toxicology and Applied Pharmacology. 254 (3), pp. 221-228. https://doi.org/10.1016/j.taap.2011.03.016
Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria
Adetutu, A., Morgan, W. and Corcoran, O. 2011. Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria. Journal of Ethnopharmacology. 137, pp. 50-56. https://doi.org/10.1016/j.jep.2011.03.073
Amphetamine and pseudoephedrine cross-tolerance measured by c-Fos protein expression in brains of chronically treated rats
Ruksee, Nootchanart, Tongjaroenbuangam, Walaiporn, Casalotti, S. and Govitrapong, Piyarat 2008. Amphetamine and pseudoephedrine cross-tolerance measured by c-Fos protein expression in brains of chronically treated rats. BMC Neuroscience. 9 (99).
Secondary metabolite mapping identifies Scutellaria inhibitors of human lung cancer cells
Gao, J., Zhao, H., Hylands, P. J. and Corcoran, O. 2010. Secondary metabolite mapping identifies Scutellaria inhibitors of human lung cancer cells. Journal of Pharmaceutical and Biomedical Analysis. 53 (3), pp. 723-728. https://doi.org/10.1016/j.jpba.2010.04.019
Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria
Adetutu, A., Morgan, W. and Corcoran, O. 2010. Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria. Journal of Ethnopharmacology. 133 (1), pp. 116-119. https://doi.org/10.1016/j.jep.2010.09.011
The Learning Gains and Student Perceptions of a Second Life Virtual Lab
Cobb, Stephanie, Heaney, Rose, Corcoran, O. and Henderson-Begg, Stephanie 2009. The Learning Gains and Student Perceptions of a Second Life Virtual Lab. Bioscience Education. https://doi.org/10.3108/beej.13.5
Triterpenoid saponins from a cytotoxic root extract of Sideroxylon foetidissimum subsp. gaumeri
Sanchez-Medina, A., Stevenson, P., Habtemariam, S., Peña-Rodríguez, L. M., Corcoran, O., Mallet, A. L. and Veitch, N. C. 2009. Triterpenoid saponins from a cytotoxic root extract of Sideroxylon foetidissimum subsp. gaumeri. Phytochemistry. 70 (6), pp. 765-772. https://doi.org/10.1016/j.phytochem.2009.03.014