Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster. 11th FENS Forum of Neuroscience. Berlin, Germany 07 - 11 Jul 2018
|Authors||Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S.|
Aims: Ethanol induced behaviours are mediated via the GABA-B receptor, but mechanisms remain unclear. Drosophila melanogaster respond to alcohol by developing addiction-like behaviours such as changes to ethanol sensitivity (tolerance). The aim of this study was to determine the effect of GABA-B receptor drugs on the development of alcohol tolerance in male adult Drosophila to further elucidate the role of GABA-B receptors.
Methods: The GABA-B receptor agonist (SKF-97451) and antagonist (CGP-54626) were administered orally for 24h before a three-day assay to challenge ethanol tolerance in wild type and GABA-B receptor mutant flies. ST50, defined as 50% of flies sedated from ethanol exposure, was used as a measure of sensitivity on each day. Results are n=6 with 10 flies per assay.
Results: Both agonist and antagonist increased the ST50 in WT and GABA-B R1 KO when compared to control. Notably, the GABA-B R2 KO did not respond to either agonist or antagonist and differed significantly from the R1 KO and WT response suggesting a complex mechanism of action.
Conclusion: The data provides evidence that the GABA-B receptors are involved in modulating the process of ethanol tolerance in Drosophila melanogaster. Further work with different mutant flies and drug delivery protocols will probe the role of these receptors in alcohol addiction.
|Conference||11th FENS Forum of Neuroscience|
|Publication process dates|
|Deposited||17 Jul 2018|
|Completed||08 Jul 2018|
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