Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells

Article

Adebola, O., Corcoran, O. and Morgan, W. 2013. Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells. Food Research International. 52 (1), pp. 269-274. https://doi.org/10.1016/j.foodres.2013.03.024
AuthorsAdebola, O., Corcoran, O. and Morgan, W.
Abstract

The accepted role of dietary prebiotics is to improve probiotic survival and growth in the lower intestine, yet few studies have investigated the possibility that prebiotics may offer benefits beyond their role with probiotics. This
study investigates the protective effects of the prebiotics inulin and lactulose against cytotoxic and genotoxic effects in HT-29 human adenocarcinoma colon cells caused by human faecal water, bile acids and 4-nitroquinoline-1-oxide (4-NQO). Using the MTT assay, deoxycholic acid, lithocholic acid and faecal water 50%v/v reduced cell viability by 75, 74 and 50% respectively. In the presence of inulin and lactulose (0.5–2.0%) cell survival increased by 100% for deoxycholic acid, by 30% for lithocholic acid and by 40% for faecal water. Both faecal
water (50% v/v) and 4-NQO (1–2 g/ml) were found to be highly genotoxic using the SOS chromotest, however incubation with inulin and lactulose (2%) significantly reduced the bacterial assay SOS inducing potency (SOSIP) by
70% and 57% respectively for faecalwater and by up to 29 and 74% for 4-NQO. Similar protection against genotoxicity was observed with 4-NQO using the Ames fluctuation test. These tests show that prebiotics, in the absence of
probiotics, were able to protect mammalian cells (HT-29 cells) against cytotoxicity and reduce the genotoxicity of known mutagens in bacterial mutation assays. Reproduction of these results in vivowould suggest that the inclusion of prebiotics in diet may directly confer health benefits beyond those associatedwith probiotic survival and growth.

JournalFood Research International
Journal citation52 (1), pp. 269-274
ISSN0963-9969
Year2013
PublisherElsevier for Canadian Institute of Food Science and Technology (CIFST)
Digital Object Identifier (DOI)https://doi.org/10.1016/j.foodres.2013.03.024
Web address (URL)https://doi.org/10.1016/j.foodres.2013.03.024
Publication dates
Online21 Mar 2013
Publication process dates
Accepted12 Mar 2013
Copyright holder© 2013 Elsevier
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