Naltrexone Reverses Ethanol Preference and Protein Kinase C Activation in Drosophila melanogaster

Article


Koyyada, Rajeswari, Latchooman, Nilesh, Jonaitis, Julius, Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Naltrexone Reverses Ethanol Preference and Protein Kinase C Activation in Drosophila melanogaster. Frontiers in Physiology. 9 (175), pp. 1-7.
AuthorsKoyyada, Rajeswari, Latchooman, Nilesh, Jonaitis, Julius, Ayoub, S., Corcoran, O. and Casalotti, S.
Abstract

Alcohol use disorder (AUD) is a major health, social and economic problem for
which there are few effective treatments. The opiate antagonist naltrexone is currently prescribed clinically with mixed success. We have used naltrexone in an established behavioral assay (CAFE) in Drosophila melanogaster that measures the flies’ preference for ethanol-containing food. We have confirmed that Drosophila exposed to ethanol develop a preference toward this drug and we demonstrate that naltrexone, in a dose dependant manner, reverses the ethanol-induced ethanol preference. This effect is not permanent, as preference for alcohol returns after discontinuing naltrexone. Additionally,
naltrexone reduced the alcohol-induced increase in protein kinase C activity. These findings are of interest because they confirm that Drosophila is a useful model for studying human responses to addictive drugs. Additionally because of the lack of a closely conserved opiate system in insects, our results could either indicate that a functionally related system does exist in insects or that in insects, and potentially also in mammals, naltrexone binds to alternative sites. Identifying such sites could lead to improved treatment strategies for AUD.

Keywordsethanol; CAFE assay; PKC; opiate antagonist; Drosophila melanogaster; naltrexone
JournalFrontiers in Physiology
Journal citation9 (175), pp. 1-7
ISSN1664-042X
Year2018
PublisherFrontiers Media
Publisher's version
License
Digital Object Identifier (DOI)doi:10.3389/fphys.2018.00175
Web address (URL)https://doi.org/10.3389/fphys.2018.00175
Publication dates
Online14 Mar 2018
Publication process dates
Deposited22 May 2018
Accepted20 Feb 2018
Accepted20 Feb 2018
FunderUniversity of East London
External resourceDataSheet1
Copyright information© 2018 The authors
LicenseCC BY 4.0
Permalink -

https://repository.uel.ac.uk/item/848q5

  • 14
    total views
  • 58
    total downloads
  • 5
    views this month
  • 3
    downloads this month

Related outputs

G-protein αq gene expression plays a role in alcohol tolerance in Drosophila melanogaster
Aleyakpo, B., Umukoro, O., Kavlie, R., Ranson, D., Thompsett, A., Corcoran, O. and Casalotti, S. 2019. G-protein αq gene expression plays a role in alcohol tolerance in Drosophila melanogaster. Brain and Neuroscience Advances. 3.
Jigsaw Recovery: The Spatio-temporalities of Alcohol Abuse and Recovery in a Non-interventionist, Peer-led Service
Kougiali, Z., Soar, K., Pytlik, A., Brown, J., Wozniak, K., Nsumba, F., Wilkins, T., Oliva, E., Osunsanya, S., Clarke, C., Casalotti, S. and Pendry, B. 2019. Jigsaw Recovery: The Spatio-temporalities of Alcohol Abuse and Recovery in a Non-interventionist, Peer-led Service. Alcoholism Treatment Quarterly.
From Scutellaria barbata to BZL101 in Cancer Patients: Phytochemistry, Pharmacology, and Clinical Evidence
Gao, J., Yin, W. and Corcoran, O. 2019. From Scutellaria barbata to BZL101 in Cancer Patients: Phytochemistry, Pharmacology, and Clinical Evidence. Natural Product Communications. 14 (10).
Loss of hypothermic and anti-pyretic action of paracetamol in cyclooxygenase-1 knockout mice is indicative of inhibition of cyclooxygenase-1 variant enzymes
Ayoub, S. and Flower, R. J. 2019. Loss of hypothermic and anti-pyretic action of paracetamol in cyclooxygenase-1 knockout mice is indicative of inhibition of cyclooxygenase-1 variant enzymes. European Journal of Pharmacology.
The Role of Oestrogen Receptor Beta (ERβ) in the Aetiology and Treatment of Type 2 Diabetes Mellitus
Ofosu, W. A., Mohamed, D., Corcoran, O. and Ojo, O. 2019. The Role of Oestrogen Receptor Beta (ERβ) in the Aetiology and Treatment of Type 2 Diabetes Mellitus. Current Diabetes Reviews. 15 (2), pp. 100-104.
Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2019. Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol. Addiction Biology.
Role of GABAB receptors in ethanol induced behaviours
Ranson, D., Ayoub, S. and Casalotti, S. 2017. Role of GABAB receptors in ethanol induced behaviours. pA2 Online: Proceedings of the British Pharmacological Society. 18 (1).
Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster. 11th FENS Forum of Neuroscience. Berlin, Germany 07 - 11 Jul 2018
Identification and preliminary structure-activity relationship studies of novel pyridyl sulfonamides as potential Chagas disease therapeutic agents
Peres, Raiza Brandão, Ullah, Asma Inam, de Almeida Fiuza, Ludmila Ferreira, Silva, Patricia Bernardino, Batista, Marcos M., Corcoran, O., Reddy, T. and de Nazaré Correia Soeiro, Maria 2018. Identification and preliminary structure-activity relationship studies of novel pyridyl sulfonamides as potential Chagas disease therapeutic agents. Bioorganic & Medicinal Chemistry Letters. 28 (11), pp. 2018-2022.
Role of gamma-aminobutyric acid-B receptors in ethanol induced behaviours
Ranson, D., Ayoub, S. and Casalotti, S. 2017. Role of gamma-aminobutyric acid-B receptors in ethanol induced behaviours. Society for the Study of Addiction Conference. Newcastle, UK 08 - 10 Nov 2017
Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects (Study Data)
Dawkins, L., Kimber, C., Doig, Mira, Feyerabend, Colin and Corcoran, O. 2018. Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects (Study Data). The University of East London. doi:10.15123/DATA.00000223
Compensatory Puffing With Lower Nicotine Concentration E-liquids Increases Carbonyl Exposure in E-cigarette Aerosols
Kośmider, Leon, Kimber, C., Kurek, Jolanta, Corcoran, O. and Dawkins, Lynne E. 2018. Compensatory Puffing With Lower Nicotine Concentration E-liquids Increases Carbonyl Exposure in E-cigarette Aerosols. Nicotine & Tobacco Research. 20 (8), pp. 998-1003.
First evidence of the conversion of paracetamol to AM404 in human cerebrospinal fluid
Sharma,, Chhaya V., Long, Jamie H., Shah, Seema, Rahman, Junia, Perrett, David, Ayoub, S. and Mehta, Vivek 2017. First evidence of the conversion of paracetamol to AM404 in human cerebrospinal fluid. Pharmacology 2017. Queen Elizabeth II Conference Centre, London, UK 11 - 13 Dec 2017
The requirement of p38-MAPKinase and peroxisome proliferator-activated receptor-g for the induction of cyclooxygenase-2 by interleukin-4 drives macrophages into an anti-inflammatory phenotype
Manoj, Sivanjaa, Morgan, W. and Ayoub, S. 2017. The requirement of p38-MAPKinase and peroxisome proliferator-activated receptor-g for the induction of cyclooxygenase-2 by interleukin-4 drives macrophages into an anti-inflammatory phenotype. Pharmacology 2017. Queen Elizabeth II Conference Centre, London, UK 11 - 13 Dec 2017
The perception of Pharmacology among undergraduate students at the University of East London
Deshai, Devyani and Ayoub, S. 2017. The perception of Pharmacology among undergraduate students at the University of East London. Pharmacology 2017. Queen Elizabeth II Conference Centre, London, UK 11 - 13 Dec 2017
First evidence of the conversion of paracetamol to AM404 in human cerebrospinal fluid
Sharma, Chhaya, Long, Jamie H, Shah, Seema, Rahman, Junia, Perrett, David, Ayoub, S. and Mehta, Vivek 2017. First evidence of the conversion of paracetamol to AM404 in human cerebrospinal fluid. Journal of Pain Research. 10, pp. 2703-2709.
Synbiotics: the impact of potential prebiotics inulin, lactulose and lactobionic acid on the survival and growth of lactobacilli probiotics
Adebola, Oluwakemi Obasola, Corcoran, O. and Morgan, Winston A. 2014. Synbiotics: the impact of potential prebiotics inulin, lactulose and lactobionic acid on the survival and growth of lactobacilli probiotics. Journal of Functional Foods. 10, pp. 75-84.
Silymarin content in Silybum marianum extracts as a biomarker for the quality of commercial tinctures
Pendry, B., Kemp, Virginia, Hughes, Michael J., Freeman, Julia, Nuhu, Hanny K., Sanchez-Medina, Alberto, Corcoran, O. and Galante, Eva 2017. Silymarin content in Silybum marianum extracts as a biomarker for the quality of commercial tinctures. Journal of Herbal Medicine. 10, pp. 31-36.
Construction of cell models for drug-receptor pharmacology activities for schools outreach: Generating awareness and interest in pharmacology in the younger generations
Ayoub, S., Cooper, Chelsey, Nunes, Fernando and Ruocco, Ray 2016. Construction of cell models for drug-receptor pharmacology activities for schools outreach: Generating awareness and interest in pharmacology in the younger generations. Pharmacology 2015. Queen Elizabeth Conference Centre, London 15 - 17 Dec 2015 pA2 Online British Pharmacological Society.
Characterisation of the signalling mechanisms involved in the induction of cyclooxygenase-2 by interleukin-4
Sivanjaa Manoj, Manoj, Morgan, W. and Ayoub, S. 2016. Characterisation of the signalling mechanisms involved in the induction of cyclooxygenase-2 by interleukin-4. PHARMACOLOGY 2016. The Queen Elizabeth II Conference Centre, London 13 - 15 Dec 2016 London British Pharmacological Society.
The Role of G Protein In Alcohol Related Behaviours Using Drosophila melanogaster As A Model
Aleyakpo, B., Umokoro, Oghenetega, Corcoran, O. and Casalotti, S. 2015. The Role of G Protein In Alcohol Related Behaviours Using Drosophila melanogaster As A Model. British Pharmacological Society Annual meeting. London Dec 2015 PA2 online British Pharmacological Society. pp. 307-307
Ethanol-induced G-protein subunit expression changes in D2 receptor deficient Drosophila
Umokoro, Oghenetega, Corcoran, O., Thompsett, Andrew and Casalotti, S. 2015. Ethanol-induced G-protein subunit expression changes in D2 receptor deficient Drosophila. British Neuroscience Association Festival 2015. Edinburgh, UK 12 - 15 Apr 2015 British Neuroscience Association. pp. 297-297
Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects
Dawkins, Lynne E., Kimber, Catherine F., Doig, Mira, Feyerabend, Colin and Corcoran, O. 2016. Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects. Psychopharmacology. 233 (15), pp. 2933-2941.
Salvinorin A content in legal high products of Salvia divinorum sold in Mexico
Hernández-Bello, Rafael, García-Rodríguez, Rosa Virginia, García-Sosa, Karlina, Peña-Rodríguez, Luis Manuel, Vázquez-Hernández, Maribel, Ramos-Morales, Fernando Rafael, Corcoran, O. and Alberto, Sánchez-Medina 2015. Salvinorin A content in legal high products of Salvia divinorum sold in Mexico. Forensic Science International. 249 (April), pp. 197-201.
Hit Discovery from Natural Products in Pharmaceutical R&D
Corcoran, O. 2015. Hit Discovery from Natural Products in Pharmaceutical R&D. Encyclopedia of Magnetic Resonance. 4 (2), pp. 455-462.
Acute electronic cigarette use: nicotine delivery and subjective effects in regular users
Dawkins, L. and Corcoran, O. 2014. Acute electronic cigarette use: nicotine delivery and subjective effects in regular users. Psychopharmacology. 231 (2), pp. 401-407.
Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts
Osei-Djarbeng, Samuel N., Cutler, S., Cutler, Ronald R. and Corcoran, O. 2014. Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts. European Journal of Medicinal Plants. 4 (7), pp. 835-843.
Ethanol-induced changes in G-proteins gene expression
Casalotti, S. 2013. Ethanol-induced changes in G-proteins gene expression. UEL Research and Knowledge Exchange Conference 2013. University of East London, London 26 Jun 2013 London University of East London.
New and novel uses for paracetamol
Ayoub, S. 2013. New and novel uses for paracetamol. UEL Research and Knowledge Exchange Conference 2013. University of East London, London 26 Jun 2013 London University of East London.
Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells
Adebola, O., Corcoran, O. and Morgan, W. 2013. Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells. Food Research International. 52 (1), pp. 269-274.
Anglo-Saxon pharmacopoeia revisited: a potential treasure in drug discovery
Watkins, Frances, Pendry, B., Corcoran, O. and Sanchez-Medina, Alberto 2011. Anglo-Saxon pharmacopoeia revisited: a potential treasure in drug discovery. Drug Discovery Today. 16 (23-24), pp. 1069-1075.
β3-integrin is required for differentiation in OC-2 cells derived from mammalian embryonic inner ear
Brunetta, Ivan, Casalotti, S., Hart, Ian R, Forge, Andrew and Reynolds, Louise E 2012. β3-integrin is required for differentiation in OC-2 cells derived from mammalian embryonic inner ear. BMC Cell Biology. 13 (1), p. 5.
Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing formulations in 10th century England
Watkins, F., Pendry, B., Sanchez-Medina, A. and Corcoran, O. 2012. Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing formulations in 10th century England. Journal of Ethnopharmacology. 144 (2), pp. 408-415.
Antimicrobial and anti-quorum sensing activities of some Ghanaian medicinal plants
Osei-Djarbeng, S., Corcoran, O., Cutler, S. and Cutler, R. R. 2012. Antimicrobial and anti-quorum sensing activities of some Ghanaian medicinal plants. International Journal of Indigenous Medicinal Plants. 28 (10), pp. 221-226.
The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells
Gao, J., Morgan, W., Sanchez-Medina, A. and Corcoran, O. 2011. The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells. Toxicology and Applied Pharmacology. 254 (3), pp. 221-228.
Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria
Adetutu, A., Morgan, W. and Corcoran, O. 2011. Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria. Journal of Ethnopharmacology. 137, pp. 50-56.
Amphetamine and pseudoephedrine cross-tolerance measured by c-Fos protein expression in brains of chronically treated rats
Ruksee, Nootchanart, Tongjaroenbuangam, Walaiporn, Casalotti, S. and Govitrapong, Piyarat 2008. Amphetamine and pseudoephedrine cross-tolerance measured by c-Fos protein expression in brains of chronically treated rats. BMC Neuroscience. 9 (99).
Secondary metabolite mapping identifies Scutellaria inhibitors of human lung cancer cells
Gao, J., Zhao, H., Hylands, P. J. and Corcoran, O. 2010. Secondary metabolite mapping identifies Scutellaria inhibitors of human lung cancer cells. Journal of Pharmaceutical and Biomedical Analysis. 53 (3), pp. 723-728.
Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria
Adetutu, A., Morgan, W. and Corcoran, O. 2010. Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria. Journal of Ethnopharmacology. 133 (1), pp. 116-119.
The Learning Gains and Student Perceptions of a Second Life Virtual Lab
Cobb, Stephanie, Heaney, Rose, Corcoran, O. and Henderson-Begg, Stephanie 2009. The Learning Gains and Student Perceptions of a Second Life Virtual Lab. Bioscience Education.
Triterpenoid saponins from a cytotoxic root extract of Sideroxylon foetidissimum subsp. gaumeri
Sanchez-Medina, A., Stevenson, P., Habtemariam, S., Peña-Rodríguez, L. M., Corcoran, O., Mallet, A. L. and Veitch, N. C. 2009. Triterpenoid saponins from a cytotoxic root extract of Sideroxylon foetidissimum subsp. gaumeri. Phytochemistry. 70 (6), pp. 765-772.