The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells

Article


Gao, J., Morgan, W., Sanchez-Medina, A. and Corcoran, O. 2011. The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells. Toxicology and Applied Pharmacology. 254 (3), pp. 221-228.
AuthorsGao, J., Morgan, W., Sanchez-Medina, A. and Corcoran, O.
Abstract

Despite a lack of scientific authentication, Scutellaria baicalensis is clinically used in Chinese medicine as a traditional adjuvant to chemotherapy of lung cancer. In this study, cytotoxicity assays demonstrated that crude ethanolic extracts of S. baicalensis were selectively toxic to human lung cancer cell lines A549, SK-LU-1 and SK-MES-1 compared with normal human lung fibroblasts. The active compounds baicalin, baicalein and wogonin did not exhibit such selectivity. Following exposure to the crude extracts, cellular protein expression
in the cancer cell lines was assessed using 2D gel electrophoresis coupled with MALDI-TOF-MS/Protein Fingerprinting. The altered protein expression indicated that cell growth arrest and apoptosis were potential mechanisms of cytotoxicity. These observations were supported by PI staining cell cycle analysis using flow cytometry and Annexin-V apoptotic analysis by fluorescence microscopy of cancer cells treated with the crude extract and pure active compounds. Moreover, specific immunoblotting identification showed the
decreased expression of cyclin A results in the S phase arrest of A549 whereas the G₀/G₁ phase arrest in SKMES-1 cells results from the decreased expression of cyclin D1. Following treatment, increased expression in the cancer cells of key proteins related to the enhancement of apoptosis was observed for p53 and Bax. These results provide further insight into the molecular mechanisms underlying the clinical use of this herb as an adjuvant to lung cancer therapy.

JournalToxicology and Applied Pharmacology
Journal citation254 (3), pp. 221-228
ISSN0041-008X
Year2011
PublisherElsevier for Academic Press
Digital Object Identifier (DOI)doi:10.1016/j.taap.2011.03.016
Web address (URL)https://doi.org/10.1016/j.taap.2011.03.016
Publication dates
Online30 Mar 2011
Publication process dates
Accepted19 Mar 2011
Copyright holder© 2011 Elsevier
Permalink -

https://repository.uel.ac.uk/item/86y83

  • 7
    total views
  • 0
    total downloads
  • 5
    views this month
  • 0
    downloads this month

Related outputs

From Scutellaria barbata to BZL101 in Cancer Patients: Phytochemistry, Pharmacology, and Clinical Evidence
Gao, J., Yin, W. and Corcoran, O. 2019. From Scutellaria barbata to BZL101 in Cancer Patients: Phytochemistry, Pharmacology, and Clinical Evidence. Natural Product Communications.
The Role of Oestrogen Receptor Beta (ERβ) in the Aetiology and Treatment of Type 2 Diabetes Mellitus
Ofosu, W. A., Mohamed, D., Corcoran, O. and Ojo, O. 2019. The Role of Oestrogen Receptor Beta (ERβ) in the Aetiology and Treatment of Type 2 Diabetes Mellitus. Current Diabetes Reviews. 15 (2), pp. 100-104.
Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2019. Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol. Addiction Biology.
No black scientist has ever won a Nobel – that’s bad for science, and bad for society
Morgan, W. 2018. No black scientist has ever won a Nobel – that’s bad for science, and bad for society. The Conversation.
Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster
Ranson, D., Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Pharmacological Modulation of Alcohol Tolerance via GABA-B receptors in Drosophila melanogaster. 11th FENS Forum of Neuroscience. Berlin, Germany 07 - 11 Jul 2018
Identification and preliminary structure-activity relationship studies of novel pyridyl sulfonamides as potential Chagas disease therapeutic agents
Peres, Raiza Brandão, Ullah, Asma Inam, de Almeida Fiuza, Ludmila Ferreira, Silva, Patricia Bernardino, Batista, Marcos M., Corcoran, O., Reddy, T. and de Nazaré Correia Soeiro, Maria 2018. Identification and preliminary structure-activity relationship studies of novel pyridyl sulfonamides as potential Chagas disease therapeutic agents. Bioorganic & Medicinal Chemistry Letters. 28 (11), pp. 2018-2022.
Naltrexone Reverses Ethanol Preference and Protein Kinase C Activation in Drosophila melanogaster
Koyyada, Rajeswari, Latchooman, Nilesh, Jonaitis, Julius, Ayoub, S., Corcoran, O. and Casalotti, S. 2018. Naltrexone Reverses Ethanol Preference and Protein Kinase C Activation in Drosophila melanogaster. Frontiers in Physiology. 9 (175), pp. 1-7.
Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects (Study Data)
Dawkins, L., Kimber, C., Doig, Mira, Feyerabend, Colin and Corcoran, O. 2018. Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects (Study Data). The University of East London. doi:10.15123/DATA.00000223
Compensatory Puffing With Lower Nicotine Concentration E-liquids Increases Carbonyl Exposure in E-cigarette Aerosols
Kośmider, Leon, Kimber, C., Kurek, Jolanta, Corcoran, O. and Dawkins, Lynne E. 2018. Compensatory Puffing With Lower Nicotine Concentration E-liquids Increases Carbonyl Exposure in E-cigarette Aerosols. Nicotine & Tobacco Research. 20 (8), pp. 998-1003.
The requirement of p38-MAPKinase and peroxisome proliferator-activated receptor-g for the induction of cyclooxygenase-2 by interleukin-4 drives macrophages into an anti-inflammatory phenotype
Manoj, Sivanjaa, Morgan, W. and Ayoub, S. 2017. The requirement of p38-MAPKinase and peroxisome proliferator-activated receptor-g for the induction of cyclooxygenase-2 by interleukin-4 drives macrophages into an anti-inflammatory phenotype. Pharmacology 2017. Queen Elizabeth II Conference Centre, London, UK 11 - 13 Dec 2017
Synbiotics: the impact of potential prebiotics inulin, lactulose and lactobionic acid on the survival and growth of lactobacilli probiotics
Adebola, Oluwakemi Obasola, Corcoran, O. and Morgan, Winston A. 2014. Synbiotics: the impact of potential prebiotics inulin, lactulose and lactobionic acid on the survival and growth of lactobacilli probiotics. Journal of Functional Foods. 10, pp. 75-84.
Transformation of monocytes to macrophages by combination antiretroviral treatment
Mafuva, Christopher and Morgan, W. 2016. Transformation of monocytes to macrophages by combination antiretroviral treatment. PHARMACOLOGY 2016. The Queen Elizabeth II Conference Centre, London 13 - 15 Dec 2016
Antipyresis and the inhibition of mitochondrial function
Elegunde, Busayo, Bashir, Shazma and Morgan, W. 2016. Antipyresis and the inhibition of mitochondrial function. PHARMACOLOGY 2016. The Queen Elizabeth II Conference Centre, London 13 - 15 Dec 2016
Effect of contraceptive hormones on a nevirapine based antiretroviral combination treatment induced apoptosis in human cervical epithelial cancer cells
Mafuva, Christopher and Morgan, W. 2017. Effect of contraceptive hormones on a nevirapine based antiretroviral combination treatment induced apoptosis in human cervical epithelial cancer cells. 32nd Annual Conference on Genes & Cancer. Robinson College, University of Cambridge, Cambridge, United Kingdom 10 - 12 Apr 2017
Silymarin content in Silybum marianum extracts as a biomarker for the quality of commercial tinctures
Pendry, B., Kemp, Virginia, Hughes, Michael J., Freeman, Julia, Nuhu, Hanny K., Sanchez-Medina, Alberto, Corcoran, O. and Galante, Eva 2017. Silymarin content in Silybum marianum extracts as a biomarker for the quality of commercial tinctures. Journal of Herbal Medicine. 10, pp. 31-36.
Characterisation of the signalling mechanisms involved in the induction of cyclooxygenase-2 by interleukin-4
Sivanjaa Manoj, Manoj, Morgan, W. and Ayoub, S. 2016. Characterisation of the signalling mechanisms involved in the induction of cyclooxygenase-2 by interleukin-4. PHARMACOLOGY 2016. The Queen Elizabeth II Conference Centre, London 13 - 15 Dec 2016 London British Pharmacological Society.
The Role of G Protein In Alcohol Related Behaviours Using Drosophila melanogaster As A Model
Aleyakpo, B., Umokoro, Oghenetega, Corcoran, O. and Casalotti, S. 2015. The Role of G Protein In Alcohol Related Behaviours Using Drosophila melanogaster As A Model. British Pharmacological Society Annual meeting. London Dec 2015 PA2 online British Pharmacological Society. pp. 307-307
Ethanol-induced G-protein subunit expression changes in D2 receptor deficient Drosophila
Umokoro, Oghenetega, Corcoran, O., Thompsett, Andrew and Casalotti, S. 2015. Ethanol-induced G-protein subunit expression changes in D2 receptor deficient Drosophila. British Neuroscience Association Festival 2015. Edinburgh, UK 12 - 15 Apr 2015 British Neuroscience Association. pp. 297-297
Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects
Dawkins, Lynne E., Kimber, Catherine F., Doig, Mira, Feyerabend, Colin and Corcoran, O. 2016. Self-titration by experienced e-cigarette users: blood nicotine delivery and subjective effects. Psychopharmacology. 233 (15), pp. 2933-2941.
Evaluation of Antibacterial Activity and Phytoconstituents of the Aqueous Leaves Extract of Alchornea Cordifolia
Antepim Pesewu, George, Culter, Ronald R., Humber, David P. and Morgan, W. 2015. Evaluation of Antibacterial Activity and Phytoconstituents of the Aqueous Leaves Extract of Alchornea Cordifolia. International Scientific Research Journal. 1 (07), pp. 48-54.
Salvinorin A content in legal high products of Salvia divinorum sold in Mexico
Hernández-Bello, Rafael, García-Rodríguez, Rosa Virginia, García-Sosa, Karlina, Peña-Rodríguez, Luis Manuel, Vázquez-Hernández, Maribel, Ramos-Morales, Fernando Rafael, Corcoran, O. and Alberto, Sánchez-Medina 2015. Salvinorin A content in legal high products of Salvia divinorum sold in Mexico. Forensic Science International. 249 (April), pp. 197-201.
Hit Discovery from Natural Products in Pharmaceutical R&D
Corcoran, O. 2015. Hit Discovery from Natural Products in Pharmaceutical R&D. Encyclopedia of Magnetic Resonance. 4 (2), pp. 455-462.
Acute electronic cigarette use: nicotine delivery and subjective effects in regular users
Dawkins, L. and Corcoran, O. 2014. Acute electronic cigarette use: nicotine delivery and subjective effects in regular users. Psychopharmacology. 231 (2), pp. 401-407.
Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts
Osei-Djarbeng, Samuel N., Cutler, S., Cutler, Ronald R. and Corcoran, O. 2014. Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts. European Journal of Medicinal Plants. 4 (7), pp. 835-843.
Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells
Adebola, O., Corcoran, O. and Morgan, W. 2013. Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells. Food Research International. 52 (1), pp. 269-274.
Anglo-Saxon pharmacopoeia revisited: a potential treasure in drug discovery
Watkins, Frances, Pendry, B., Corcoran, O. and Sanchez-Medina, Alberto 2011. Anglo-Saxon pharmacopoeia revisited: a potential treasure in drug discovery. Drug Discovery Today. 16 (23-24), pp. 1069-1075.
Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing formulations in 10th century England
Watkins, F., Pendry, B., Sanchez-Medina, A. and Corcoran, O. 2012. Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing formulations in 10th century England. Journal of Ethnopharmacology. 144 (2), pp. 408-415.
Antimicrobial and anti-quorum sensing activities of some Ghanaian medicinal plants
Osei-Djarbeng, S., Corcoran, O., Cutler, S. and Cutler, R. R. 2012. Antimicrobial and anti-quorum sensing activities of some Ghanaian medicinal plants. International Journal of Indigenous Medicinal Plants. 28 (10), pp. 221-226.
Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria
Adetutu, A., Morgan, W. and Corcoran, O. 2011. Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria. Journal of Ethnopharmacology. 137, pp. 50-56.
Secondary metabolite mapping identifies Scutellaria inhibitors of human lung cancer cells
Gao, J., Zhao, H., Hylands, P. J. and Corcoran, O. 2010. Secondary metabolite mapping identifies Scutellaria inhibitors of human lung cancer cells. Journal of Pharmaceutical and Biomedical Analysis. 53 (3), pp. 723-728.
Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria
Adetutu, A., Morgan, W. and Corcoran, O. 2010. Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria. Journal of Ethnopharmacology. 133 (1), pp. 116-119.
The Learning Gains and Student Perceptions of a Second Life Virtual Lab
Cobb, Stephanie, Heaney, Rose, Corcoran, O. and Henderson-Begg, Stephanie 2009. The Learning Gains and Student Perceptions of a Second Life Virtual Lab. Bioscience Education.
Triterpenoid saponins from a cytotoxic root extract of Sideroxylon foetidissimum subsp. gaumeri
Sanchez-Medina, A., Stevenson, P., Habtemariam, S., Peña-Rodríguez, L. M., Corcoran, O., Mallet, A. L. and Veitch, N. C. 2009. Triterpenoid saponins from a cytotoxic root extract of Sideroxylon foetidissimum subsp. gaumeri. Phytochemistry. 70 (6), pp. 765-772.